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Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398
Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus se...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Springer-Verlag
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797422/ https://www.ncbi.nlm.nih.gov/pubmed/19795142 http://dx.doi.org/10.1007/s10096-009-0816-3 |
| _version_ | 1782175617418002432 |
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| author | van Wamel, W. J. B. Hansenová Maňásková, S. Fluit, A. C. Verbrugh, H. de Neeling, A. J. van Duijkeren, E. van Belkum, A. |
| author_facet | van Wamel, W. J. B. Hansenová Maňásková, S. Fluit, A. C. Verbrugh, H. de Neeling, A. J. van Duijkeren, E. van Belkum, A. |
| author_sort | van Wamel, W. J. B. |
| collection | PubMed |
| description | Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity. |
| format | Text |
| id | pubmed-2797422 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2009 |
| publisher | Springer-Verlag |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-27974222009-12-29 Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 van Wamel, W. J. B. Hansenová Maňásková, S. Fluit, A. C. Verbrugh, H. de Neeling, A. J. van Duijkeren, E. van Belkum, A. Eur J Clin Microbiol Infect Dis Brief Report Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity. Springer-Verlag 2009-10-02 2010 /pmc/articles/PMC2797422/ /pubmed/19795142 http://dx.doi.org/10.1007/s10096-009-0816-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
| spellingShingle | Brief Report van Wamel, W. J. B. Hansenová Maňásková, S. Fluit, A. C. Verbrugh, H. de Neeling, A. J. van Duijkeren, E. van Belkum, A. Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title | Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title_full | Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title_fullStr | Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title_full_unstemmed | Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title_short | Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 |
| title_sort | short term micro-evolution and pcr-detection of methicillin-resistant and -susceptible staphylococcus aureus sequence type 398 |
| topic | Brief Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797422/ https://www.ncbi.nlm.nih.gov/pubmed/19795142 http://dx.doi.org/10.1007/s10096-009-0816-3 |
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