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Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells

BACKGROUND: Mitochondria can sense signals linked to variations in energy demand to regulate nuclear gene expression. This retrograde signaling pathway is presumed to be involved in the regulation of myoblast proliferation and differentiation. Rhabdomyosarcoma cells are characterized by their failur...

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Autores principales: Jahnke, Vanessa E., Sabido, Odile, Defour, Aurélia, Castells, Josiane, Lefai, Etienne, Roussel, Damien, Freyssenet, Damien
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797644/
https://www.ncbi.nlm.nih.gov/pubmed/20072609
http://dx.doi.org/10.1371/journal.pone.0008637
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author Jahnke, Vanessa E.
Sabido, Odile
Defour, Aurélia
Castells, Josiane
Lefai, Etienne
Roussel, Damien
Freyssenet, Damien
author_facet Jahnke, Vanessa E.
Sabido, Odile
Defour, Aurélia
Castells, Josiane
Lefai, Etienne
Roussel, Damien
Freyssenet, Damien
author_sort Jahnke, Vanessa E.
collection PubMed
description BACKGROUND: Mitochondria can sense signals linked to variations in energy demand to regulate nuclear gene expression. This retrograde signaling pathway is presumed to be involved in the regulation of myoblast proliferation and differentiation. Rhabdomyosarcoma cells are characterized by their failure to both irreversibly exit the cell cycle and complete myogenic differentiation. However, it is currently unknown whether mitochondria are involved in the failure of rhabdomyosarcoma cells to differentiate. METHODOLOGY/PRINCIPAL FINDINGS: Mitochondrial biogenesis and metabolism were studied in rat L6E9 myoblasts and R1H rhabdomyosacoma cells during the cell cycle and after 36 hours of differentiation. Using a combination of flow cytometry, polarographic and molecular analyses, we evidenced a marked decrease in the cardiolipin content of R1H cells cultured in growth and differentiation media, together with a significant increase in the content of mitochondrial biogenesis factors and mitochondrial respiratory chain proteins. Altogether, these data indicate that the mitochondrial inner membrane composition and the overall process of mitochondrial biogenesis are markedly altered in R1H cells. Importantly, the dysregulation of protein-to-cardiolipin ratio was associated with major deficiencies in both basal and maximal mitochondrial respiration rates. This deficiency in mitochondrial respiration probably contributes to the inability of R1H cells to decrease mitochondrial H(2)O(2) level at the onset of differentiation. CONCLUSION/SIGNIFICANCE: A defect in the regulation of mitochondrial biogenesis and mitochondrial metabolism may thus be an epigenetic mechanism that may contribute to the tumoral behavior of R1H cells. Our data underline the importance of mitochondria in the regulation of myogenic differentiation.
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spelling pubmed-27976442010-01-14 Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells Jahnke, Vanessa E. Sabido, Odile Defour, Aurélia Castells, Josiane Lefai, Etienne Roussel, Damien Freyssenet, Damien PLoS One Research Article BACKGROUND: Mitochondria can sense signals linked to variations in energy demand to regulate nuclear gene expression. This retrograde signaling pathway is presumed to be involved in the regulation of myoblast proliferation and differentiation. Rhabdomyosarcoma cells are characterized by their failure to both irreversibly exit the cell cycle and complete myogenic differentiation. However, it is currently unknown whether mitochondria are involved in the failure of rhabdomyosarcoma cells to differentiate. METHODOLOGY/PRINCIPAL FINDINGS: Mitochondrial biogenesis and metabolism were studied in rat L6E9 myoblasts and R1H rhabdomyosacoma cells during the cell cycle and after 36 hours of differentiation. Using a combination of flow cytometry, polarographic and molecular analyses, we evidenced a marked decrease in the cardiolipin content of R1H cells cultured in growth and differentiation media, together with a significant increase in the content of mitochondrial biogenesis factors and mitochondrial respiratory chain proteins. Altogether, these data indicate that the mitochondrial inner membrane composition and the overall process of mitochondrial biogenesis are markedly altered in R1H cells. Importantly, the dysregulation of protein-to-cardiolipin ratio was associated with major deficiencies in both basal and maximal mitochondrial respiration rates. This deficiency in mitochondrial respiration probably contributes to the inability of R1H cells to decrease mitochondrial H(2)O(2) level at the onset of differentiation. CONCLUSION/SIGNIFICANCE: A defect in the regulation of mitochondrial biogenesis and mitochondrial metabolism may thus be an epigenetic mechanism that may contribute to the tumoral behavior of R1H cells. Our data underline the importance of mitochondria in the regulation of myogenic differentiation. Public Library of Science 2010-01-08 /pmc/articles/PMC2797644/ /pubmed/20072609 http://dx.doi.org/10.1371/journal.pone.0008637 Text en Jahnke et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jahnke, Vanessa E.
Sabido, Odile
Defour, Aurélia
Castells, Josiane
Lefai, Etienne
Roussel, Damien
Freyssenet, Damien
Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title_full Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title_fullStr Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title_full_unstemmed Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title_short Evidence for Mitochondrial Respiratory Deficiency in Rat Rhabdomyosarcoma Cells
title_sort evidence for mitochondrial respiratory deficiency in rat rhabdomyosarcoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797644/
https://www.ncbi.nlm.nih.gov/pubmed/20072609
http://dx.doi.org/10.1371/journal.pone.0008637
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