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Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797812/ https://www.ncbi.nlm.nih.gov/pubmed/20003261 http://dx.doi.org/10.1186/1756-0500-2-245 |
Sumario: | BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS: We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions. CONCLUSION: This enables a fast and efficient determination of IL-6 (-597/-572/-174)haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts. |
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