Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes

BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS...

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Detalles Bibliográficos
Autores principales: Müller-Steinhardt, Michael, Schulte, Friederike, Klüter, Harald, Bugert, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797812/
https://www.ncbi.nlm.nih.gov/pubmed/20003261
http://dx.doi.org/10.1186/1756-0500-2-245
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author Müller-Steinhardt, Michael
Schulte, Friederike
Klüter, Harald
Bugert, Peter
author_facet Müller-Steinhardt, Michael
Schulte, Friederike
Klüter, Harald
Bugert, Peter
author_sort Müller-Steinhardt, Michael
collection PubMed
description BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS: We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions. CONCLUSION: This enables a fast and efficient determination of IL-6 (-597/-572/-174)haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts.
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spelling pubmed-27978122009-12-25 Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes Müller-Steinhardt, Michael Schulte, Friederike Klüter, Harald Bugert, Peter BMC Res Notes Short Report BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS: We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions. CONCLUSION: This enables a fast and efficient determination of IL-6 (-597/-572/-174)haplotypes in clinical diagnosis and further evaluation of IL-6 promoter polymorphisms in larger patient cohorts. BioMed Central 2009-12-10 /pmc/articles/PMC2797812/ /pubmed/20003261 http://dx.doi.org/10.1186/1756-0500-2-245 Text en Copyright ©2009 Müller-Steinhardt et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Müller-Steinhardt, Michael
Schulte, Friederike
Klüter, Harald
Bugert, Peter
Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title_full Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title_fullStr Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title_full_unstemmed Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title_short Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes
title_sort optimized pcr with sequence specific primers (pcr-ssp) for fast and efficient determination of interleukin-6 promoter (-597/-572/-174)haplotypes
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797812/
https://www.ncbi.nlm.nih.gov/pubmed/20003261
http://dx.doi.org/10.1186/1756-0500-2-245
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