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Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes

BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS...

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Detalles Bibliográficos
Autores principales:	Müller-Steinhardt, Michael, Schulte, Friederike, Klüter, Harald, Bugert, Peter
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797812/ https://www.ncbi.nlm.nih.gov/pubmed/20003261 http://dx.doi.org/10.1186/1756-0500-2-245

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