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Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells

Somatic neural and neural crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because of practical considerations such as inadequate accessibility of the source material, the application of neural crest stem cells is strictly limited. The secon...

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Autores principales: Widera, Darius, Zander, Christin, Heidbreder, Meike, Kasperek, Yvonne, Noll, Thomas, Seitz, Oliver, Saldamli, Belma, Sudhoff, Holger, Sader, Robert, Kaltschmidt, Christian, Kaltschmidt, Barbara
Formato: Texto
Lenguaje:English
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798069/
https://www.ncbi.nlm.nih.gov/pubmed/19544446
http://dx.doi.org/10.1002/stem.104
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author Widera, Darius
Zander, Christin
Heidbreder, Meike
Kasperek, Yvonne
Noll, Thomas
Seitz, Oliver
Saldamli, Belma
Sudhoff, Holger
Sader, Robert
Kaltschmidt, Christian
Kaltschmidt, Barbara
author_facet Widera, Darius
Zander, Christin
Heidbreder, Meike
Kasperek, Yvonne
Noll, Thomas
Seitz, Oliver
Saldamli, Belma
Sudhoff, Holger
Sader, Robert
Kaltschmidt, Christian
Kaltschmidt, Barbara
author_sort Widera, Darius
collection PubMed
description Somatic neural and neural crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because of practical considerations such as inadequate accessibility of the source material, the application of neural crest stem cells is strictly limited. The secondary palate is a highly regenerative and heavily innervated tissue, which develops embryonically under direct contribution of neural crest cells. Here, we describe for the first time the presence of nestin-positive neural crest-related stem cells within Meissner corpuscles and Merkel cell-neurite complexes located in the hard palate of adult Wistar rats. After isolation, palatal neural crest-related stem cells (pNC-SCs) were cultivated in the presence of epidermal growth factor and fibroblast growth factor under serum-free conditions, resulting in large amounts of neurospheres. We used immunocytochemical techniques and reverse transcriptase-polymerase chain reaction to assess the expression profile of pNC-SCs. In addition to the expression of neural crest stem cell markers such as Nestin, Sox2, and p75, we detected the expression of Klf4, Oct4, and c-Myc. pNC-SCs differentiated efficiently into neuronal and glial cells. Finally, we investigated the potential expression of stemness markers within the human palate. We identified expression of stem cell markers nestin and CD133 and the transcription factors needed for reprogramming of somatic cells into pluripotent cells: Sox2, Oct4, Klf4, and c-Myc. These data show that cells isolated from palatal rugae form neurospheres, are highly plastic, and express neural crest stem cell markers. In addition, pNC-SCs may have the ability to differentiate into functional neurons and glial cells, serving as a starting point for therapeutic studies. Stem Cells 2009;27:1899–1910
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spelling pubmed-27980692009-12-29 Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells Widera, Darius Zander, Christin Heidbreder, Meike Kasperek, Yvonne Noll, Thomas Seitz, Oliver Saldamli, Belma Sudhoff, Holger Sader, Robert Kaltschmidt, Christian Kaltschmidt, Barbara Stem Cells Tissue-Specific Stem Cells Somatic neural and neural crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because of practical considerations such as inadequate accessibility of the source material, the application of neural crest stem cells is strictly limited. The secondary palate is a highly regenerative and heavily innervated tissue, which develops embryonically under direct contribution of neural crest cells. Here, we describe for the first time the presence of nestin-positive neural crest-related stem cells within Meissner corpuscles and Merkel cell-neurite complexes located in the hard palate of adult Wistar rats. After isolation, palatal neural crest-related stem cells (pNC-SCs) were cultivated in the presence of epidermal growth factor and fibroblast growth factor under serum-free conditions, resulting in large amounts of neurospheres. We used immunocytochemical techniques and reverse transcriptase-polymerase chain reaction to assess the expression profile of pNC-SCs. In addition to the expression of neural crest stem cell markers such as Nestin, Sox2, and p75, we detected the expression of Klf4, Oct4, and c-Myc. pNC-SCs differentiated efficiently into neuronal and glial cells. Finally, we investigated the potential expression of stemness markers within the human palate. We identified expression of stem cell markers nestin and CD133 and the transcription factors needed for reprogramming of somatic cells into pluripotent cells: Sox2, Oct4, Klf4, and c-Myc. These data show that cells isolated from palatal rugae form neurospheres, are highly plastic, and express neural crest stem cell markers. In addition, pNC-SCs may have the ability to differentiate into functional neurons and glial cells, serving as a starting point for therapeutic studies. Stem Cells 2009;27:1899–1910 Wiley Subscription Services, Inc., A Wiley Company 2009-08 /pmc/articles/PMC2798069/ /pubmed/19544446 http://dx.doi.org/10.1002/stem.104 Text en Copyright © 2009 AlphaMed Press http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Tissue-Specific Stem Cells
Widera, Darius
Zander, Christin
Heidbreder, Meike
Kasperek, Yvonne
Noll, Thomas
Seitz, Oliver
Saldamli, Belma
Sudhoff, Holger
Sader, Robert
Kaltschmidt, Christian
Kaltschmidt, Barbara
Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title_full Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title_fullStr Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title_full_unstemmed Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title_short Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells
title_sort adult palatum as a novel source of neural crest-related stem cells
topic Tissue-Specific Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798069/
https://www.ncbi.nlm.nih.gov/pubmed/19544446
http://dx.doi.org/10.1002/stem.104
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