Cargando…

Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat

BACKGROUND: Chromium picolinate (Cr(pic)3) is advocated as adjuvant therapy for impaired glycemic control, despite concerns for DNA damage. Potential toxicity of Cr(pic)3 should be greater for the kidney that accumulates chromium. Therefore, we tested the hypothesis that Cr(pic)3 treatment of obese...

Descripción completa

Detalles Bibliográficos
Autores principales: Mozaffari, Mahmood S, AbdelSayed, Rafik, Liu, Jun Yao, Wimborne, Hereward, El-Remessy, Azza, El-Marakby, Ahmed
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799425/
https://www.ncbi.nlm.nih.gov/pubmed/20003253
http://dx.doi.org/10.1186/1743-7075-6-51
_version_ 1782175781383831552
author Mozaffari, Mahmood S
AbdelSayed, Rafik
Liu, Jun Yao
Wimborne, Hereward
El-Remessy, Azza
El-Marakby, Ahmed
author_facet Mozaffari, Mahmood S
AbdelSayed, Rafik
Liu, Jun Yao
Wimborne, Hereward
El-Remessy, Azza
El-Marakby, Ahmed
author_sort Mozaffari, Mahmood S
collection PubMed
description BACKGROUND: Chromium picolinate (Cr(pic)3) is advocated as adjuvant therapy for impaired glycemic control, despite concerns for DNA damage. Potential toxicity of Cr(pic)3 should be greater for the kidney that accumulates chromium. Therefore, we tested the hypothesis that Cr(pic)3 treatment of obese Zucker rats (OZR) exacerbates renal abnormalities associated with dysglycemia. METHODS: Male OZR were treated with diets lacking or containing 5 and 10 mg/kg of chromium, as Cr(pic)3, for 20 weeks; lean Zucker rats (LZR) served as controls. Glycemic and renal effects of Cr(pic)3 were determined in the context of indices of oxidative stress and inflammation. RESULTS: The OZR displayed increased fasting plasma glucose and insulin in association with enlarged pancreatic islets exhibiting collagen and periodic acid Schiff-positive deposits compared to LZR; Cr(pic)3 treatment did not affect these parameters. The OZR, irrespective of Cr(pic)3, excreted more albumin than LZR. Also, other indices of renal function or histopathology were not affected by Cr(pic)3 treatment. Urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG), an index of oxidative DNA damage, was greater in the OZR than LZR; dietary Cr(pic)3 treatment attenuated 8-OHdG excretion. However, immunostaining of kidney for 8-OHdG revealed similar staining pattern and intensity, despite significant renal accumulation of chromium in Cr(pic)3-treated groups. Finally, increased renal nitrotyrosine and cyclooxygenase-2 levels and urinary excretion of monocyte chemoattractant protein-1 of OZR were partially reversed by Cr(pic)3 treatment. CONCLUSION: Dietary Cr(pic)3 treatment of OZR does not beneficially influence glycemic status or increase the risk for oxidative DNA damage; rather, the treatment attenuates indices of oxidative stress and inflammation.
format Text
id pubmed-2799425
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-27994252009-12-30 Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat Mozaffari, Mahmood S AbdelSayed, Rafik Liu, Jun Yao Wimborne, Hereward El-Remessy, Azza El-Marakby, Ahmed Nutr Metab (Lond) Research BACKGROUND: Chromium picolinate (Cr(pic)3) is advocated as adjuvant therapy for impaired glycemic control, despite concerns for DNA damage. Potential toxicity of Cr(pic)3 should be greater for the kidney that accumulates chromium. Therefore, we tested the hypothesis that Cr(pic)3 treatment of obese Zucker rats (OZR) exacerbates renal abnormalities associated with dysglycemia. METHODS: Male OZR were treated with diets lacking or containing 5 and 10 mg/kg of chromium, as Cr(pic)3, for 20 weeks; lean Zucker rats (LZR) served as controls. Glycemic and renal effects of Cr(pic)3 were determined in the context of indices of oxidative stress and inflammation. RESULTS: The OZR displayed increased fasting plasma glucose and insulin in association with enlarged pancreatic islets exhibiting collagen and periodic acid Schiff-positive deposits compared to LZR; Cr(pic)3 treatment did not affect these parameters. The OZR, irrespective of Cr(pic)3, excreted more albumin than LZR. Also, other indices of renal function or histopathology were not affected by Cr(pic)3 treatment. Urinary excretion of 8-hydroxydeoxyguanosine (8-OHdG), an index of oxidative DNA damage, was greater in the OZR than LZR; dietary Cr(pic)3 treatment attenuated 8-OHdG excretion. However, immunostaining of kidney for 8-OHdG revealed similar staining pattern and intensity, despite significant renal accumulation of chromium in Cr(pic)3-treated groups. Finally, increased renal nitrotyrosine and cyclooxygenase-2 levels and urinary excretion of monocyte chemoattractant protein-1 of OZR were partially reversed by Cr(pic)3 treatment. CONCLUSION: Dietary Cr(pic)3 treatment of OZR does not beneficially influence glycemic status or increase the risk for oxidative DNA damage; rather, the treatment attenuates indices of oxidative stress and inflammation. BioMed Central 2009-12-10 /pmc/articles/PMC2799425/ /pubmed/20003253 http://dx.doi.org/10.1186/1743-7075-6-51 Text en Copyright ©2009 Mozaffari et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mozaffari, Mahmood S
AbdelSayed, Rafik
Liu, Jun Yao
Wimborne, Hereward
El-Remessy, Azza
El-Marakby, Ahmed
Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title_full Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title_fullStr Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title_full_unstemmed Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title_short Effects of chromium picolinate on glycemic control and kidney of the obese Zucker rat
title_sort effects of chromium picolinate on glycemic control and kidney of the obese zucker rat
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799425/
https://www.ncbi.nlm.nih.gov/pubmed/20003253
http://dx.doi.org/10.1186/1743-7075-6-51
work_keys_str_mv AT mozaffarimahmoods effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat
AT abdelsayedrafik effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat
AT liujunyao effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat
AT wimbornehereward effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat
AT elremessyazza effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat
AT elmarakbyahmed effectsofchromiumpicolinateonglycemiccontrolandkidneyoftheobesezuckerrat