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The maize root stem cell niche: a partnership between two sister cell populations

Using transcript profile analysis, we explored the nature of the stem cell niche in roots of maize (Zea mays). Toward assessing a role for specific genes in the establishment and maintenance of the niche, we perturbed the niche and simultaneously monitored the spatial expression patterns of genes hy...

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Detalles Bibliográficos
Autores principales: Jiang, Keni, Zhu, Tong, Diao, Zhaoyan, Huang, Haiyan, Feldman, Lewis J.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799627/
https://www.ncbi.nlm.nih.gov/pubmed/20041334
http://dx.doi.org/10.1007/s00425-009-1059-3
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author Jiang, Keni
Zhu, Tong
Diao, Zhaoyan
Huang, Haiyan
Feldman, Lewis J.
author_facet Jiang, Keni
Zhu, Tong
Diao, Zhaoyan
Huang, Haiyan
Feldman, Lewis J.
author_sort Jiang, Keni
collection PubMed
description Using transcript profile analysis, we explored the nature of the stem cell niche in roots of maize (Zea mays). Toward assessing a role for specific genes in the establishment and maintenance of the niche, we perturbed the niche and simultaneously monitored the spatial expression patterns of genes hypothesized as essential. Our results allow us to quantify and localize gene activities to specific portions of the niche: to the quiescent center (QC) or the proximal meristem (PM), or to both. The data point to molecular, biochemical and physiological processes associated with the specification and maintenance of the niche, and include reduced expression of metabolism-, redox- and certain cell cycle-associated transcripts in the QC, enrichment of auxin-associated transcripts within the entire niche, controls for the state of differentiation of QC cells, a role for cytokinins specifically in the PM portion of the niche, processes (repair machinery) for maintaining DNA integrity and a role for gene silencing in niche stabilization. To provide additional support for the hypothesized roles of the above-mentioned and other transcripts in niche specification, we overexpressed, in Arabidopsis, homologs of representative genes (eight) identified as highly enriched or reduced in the maize root QC. We conclude that the coordinated changes in expression of auxin-, redox-, cell cycle- and metabolism-associated genes suggest the linkage of gene networks at the level of transcription, thereby providing additional insights into events likely associated with root stem cell niche establishment and maintenance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-009-1059-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-27996272009-12-30 The maize root stem cell niche: a partnership between two sister cell populations Jiang, Keni Zhu, Tong Diao, Zhaoyan Huang, Haiyan Feldman, Lewis J. Planta Original Article Using transcript profile analysis, we explored the nature of the stem cell niche in roots of maize (Zea mays). Toward assessing a role for specific genes in the establishment and maintenance of the niche, we perturbed the niche and simultaneously monitored the spatial expression patterns of genes hypothesized as essential. Our results allow us to quantify and localize gene activities to specific portions of the niche: to the quiescent center (QC) or the proximal meristem (PM), or to both. The data point to molecular, biochemical and physiological processes associated with the specification and maintenance of the niche, and include reduced expression of metabolism-, redox- and certain cell cycle-associated transcripts in the QC, enrichment of auxin-associated transcripts within the entire niche, controls for the state of differentiation of QC cells, a role for cytokinins specifically in the PM portion of the niche, processes (repair machinery) for maintaining DNA integrity and a role for gene silencing in niche stabilization. To provide additional support for the hypothesized roles of the above-mentioned and other transcripts in niche specification, we overexpressed, in Arabidopsis, homologs of representative genes (eight) identified as highly enriched or reduced in the maize root QC. We conclude that the coordinated changes in expression of auxin-, redox-, cell cycle- and metabolism-associated genes suggest the linkage of gene networks at the level of transcription, thereby providing additional insights into events likely associated with root stem cell niche establishment and maintenance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-009-1059-3) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-11-22 2010 /pmc/articles/PMC2799627/ /pubmed/20041334 http://dx.doi.org/10.1007/s00425-009-1059-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Jiang, Keni
Zhu, Tong
Diao, Zhaoyan
Huang, Haiyan
Feldman, Lewis J.
The maize root stem cell niche: a partnership between two sister cell populations
title The maize root stem cell niche: a partnership between two sister cell populations
title_full The maize root stem cell niche: a partnership between two sister cell populations
title_fullStr The maize root stem cell niche: a partnership between two sister cell populations
title_full_unstemmed The maize root stem cell niche: a partnership between two sister cell populations
title_short The maize root stem cell niche: a partnership between two sister cell populations
title_sort maize root stem cell niche: a partnership between two sister cell populations
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799627/
https://www.ncbi.nlm.nih.gov/pubmed/20041334
http://dx.doi.org/10.1007/s00425-009-1059-3
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