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Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles

Plant plasma membrane (pm) vesicles from mycorrhizal tobacco (Nicotiana tabacum cv. Samsun) roots were isolated with negligible fungal contamination by the aqueous two-phase partitioning technique as proven by fatty acid analysis. Palmitvaccenic acid became apparent as an appropriate indicator for f...

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Autores principales: Moche, Martin, Stremlau, Stefanie, Hecht, Lars, Göbel, Cornelia, Feussner, Ivo, Stöhr, Christine
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799628/
https://www.ncbi.nlm.nih.gov/pubmed/19937342
http://dx.doi.org/10.1007/s00425-009-1057-5
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author Moche, Martin
Stremlau, Stefanie
Hecht, Lars
Göbel, Cornelia
Feussner, Ivo
Stöhr, Christine
author_facet Moche, Martin
Stremlau, Stefanie
Hecht, Lars
Göbel, Cornelia
Feussner, Ivo
Stöhr, Christine
author_sort Moche, Martin
collection PubMed
description Plant plasma membrane (pm) vesicles from mycorrhizal tobacco (Nicotiana tabacum cv. Samsun) roots were isolated with negligible fungal contamination by the aqueous two-phase partitioning technique as proven by fatty acid analysis. Palmitvaccenic acid became apparent as an appropriate indicator for fungal membranes in root pm preparations. The pm vesicles had a low specific activity of the vanadate-sensitive ATPase and probably originated from non-infected root cells. In a phosphate-limited tobacco culture system, root colonisation by the vesicular arbuscular mycorrhizal fungus, Glomus mosseae, is inhibited by external nitrate in a dose-dependent way. However, detrimental high concentrations of 25 mM nitrate lead to the highest colonisation rate observed, indicating that the defence system of the plant is impaired. Nitric oxide formation by the pm-bound nitrite:NO reductase increased in parallel with external nitrate supply in mycorrhizal roots in comparison to the control plants, but decreased under excess nitrate. Mycorrhizal pm vesicles had roughly a twofold higher specific activity as the non-infected control plants when supplied with 10–15 mM nitrate.
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spelling pubmed-27996282009-12-30 Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles Moche, Martin Stremlau, Stefanie Hecht, Lars Göbel, Cornelia Feussner, Ivo Stöhr, Christine Planta Original Article Plant plasma membrane (pm) vesicles from mycorrhizal tobacco (Nicotiana tabacum cv. Samsun) roots were isolated with negligible fungal contamination by the aqueous two-phase partitioning technique as proven by fatty acid analysis. Palmitvaccenic acid became apparent as an appropriate indicator for fungal membranes in root pm preparations. The pm vesicles had a low specific activity of the vanadate-sensitive ATPase and probably originated from non-infected root cells. In a phosphate-limited tobacco culture system, root colonisation by the vesicular arbuscular mycorrhizal fungus, Glomus mosseae, is inhibited by external nitrate in a dose-dependent way. However, detrimental high concentrations of 25 mM nitrate lead to the highest colonisation rate observed, indicating that the defence system of the plant is impaired. Nitric oxide formation by the pm-bound nitrite:NO reductase increased in parallel with external nitrate supply in mycorrhizal roots in comparison to the control plants, but decreased under excess nitrate. Mycorrhizal pm vesicles had roughly a twofold higher specific activity as the non-infected control plants when supplied with 10–15 mM nitrate. Springer-Verlag 2009-11-25 2010 /pmc/articles/PMC2799628/ /pubmed/19937342 http://dx.doi.org/10.1007/s00425-009-1057-5 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Moche, Martin
Stremlau, Stefanie
Hecht, Lars
Göbel, Cornelia
Feussner, Ivo
Stöhr, Christine
Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title_full Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title_fullStr Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title_full_unstemmed Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title_short Effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
title_sort effect of nitrate supply and mycorrhizal inoculation on characteristics of tobacco root plasma membrane vesicles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799628/
https://www.ncbi.nlm.nih.gov/pubmed/19937342
http://dx.doi.org/10.1007/s00425-009-1057-5
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