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Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant

In an attempt to elucidate the underlying longevity-promoting mechanisms of mutants lacking SCH9, which live three times as long as wild type chronologically, we measured their time-course gene expression profiles. We interpreted their expression time differences by statistical inferences based on p...

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Autores principales: Ge, Huanying, Wei, Min, Fabrizio, Paola, Hu, Jia, Cheng, Chao, Longo, Valter D., Li, Lei M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2800218/
https://www.ncbi.nlm.nih.gov/pubmed/19880387
http://dx.doi.org/10.1093/nar/gkp849
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author Ge, Huanying
Wei, Min
Fabrizio, Paola
Hu, Jia
Cheng, Chao
Longo, Valter D.
Li, Lei M.
author_facet Ge, Huanying
Wei, Min
Fabrizio, Paola
Hu, Jia
Cheng, Chao
Longo, Valter D.
Li, Lei M.
author_sort Ge, Huanying
collection PubMed
description In an attempt to elucidate the underlying longevity-promoting mechanisms of mutants lacking SCH9, which live three times as long as wild type chronologically, we measured their time-course gene expression profiles. We interpreted their expression time differences by statistical inferences based on prior biological knowledge, and identified the following significant changes: (i) between 12 and 24 h, stress response genes were up-regulated by larger fold changes and ribosomal RNA (rRNA) processing genes were down-regulated more dramatically; (ii) mitochondrial ribosomal protein genes were not up-regulated between 12 and 60 h as wild type were; (iii) electron transport, oxidative phosphorylation and TCA genes were down-regulated early; (iv) the up-regulation of TCA and electron transport was accompanied by deep down-regulation of rRNA processing over time; and (v) rRNA processing genes were more volatile over time, and three associated cis-regulatory elements [rRNA processing element (rRPE), polymerase A and C (PAC) and glucose response element (GRE)] were identified. Deletion of AZF1, which encodes the transcriptional factor that binds to the GRE element, reversed the lifespan extension of sch9Δ. The significant alterations in these time-dependent expression profiles imply that the lack of SCH9 turns on the longevity programme that extends the lifespan through changes in metabolic pathways and protection mechanisms, particularly, the regulation of aerobic respiration and rRNA processing.
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spelling pubmed-28002182009-12-31 Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant Ge, Huanying Wei, Min Fabrizio, Paola Hu, Jia Cheng, Chao Longo, Valter D. Li, Lei M. Nucleic Acids Res Genomics In an attempt to elucidate the underlying longevity-promoting mechanisms of mutants lacking SCH9, which live three times as long as wild type chronologically, we measured their time-course gene expression profiles. We interpreted their expression time differences by statistical inferences based on prior biological knowledge, and identified the following significant changes: (i) between 12 and 24 h, stress response genes were up-regulated by larger fold changes and ribosomal RNA (rRNA) processing genes were down-regulated more dramatically; (ii) mitochondrial ribosomal protein genes were not up-regulated between 12 and 60 h as wild type were; (iii) electron transport, oxidative phosphorylation and TCA genes were down-regulated early; (iv) the up-regulation of TCA and electron transport was accompanied by deep down-regulation of rRNA processing over time; and (v) rRNA processing genes were more volatile over time, and three associated cis-regulatory elements [rRNA processing element (rRPE), polymerase A and C (PAC) and glucose response element (GRE)] were identified. Deletion of AZF1, which encodes the transcriptional factor that binds to the GRE element, reversed the lifespan extension of sch9Δ. The significant alterations in these time-dependent expression profiles imply that the lack of SCH9 turns on the longevity programme that extends the lifespan through changes in metabolic pathways and protection mechanisms, particularly, the regulation of aerobic respiration and rRNA processing. Oxford University Press 2010-01 2009-10-30 /pmc/articles/PMC2800218/ /pubmed/19880387 http://dx.doi.org/10.1093/nar/gkp849 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genomics
Ge, Huanying
Wei, Min
Fabrizio, Paola
Hu, Jia
Cheng, Chao
Longo, Valter D.
Li, Lei M.
Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title_full Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title_fullStr Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title_full_unstemmed Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title_short Comparative analyses of time-course gene expression profiles of the long-lived sch9Δ mutant
title_sort comparative analyses of time-course gene expression profiles of the long-lived sch9δ mutant
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2800218/
https://www.ncbi.nlm.nih.gov/pubmed/19880387
http://dx.doi.org/10.1093/nar/gkp849
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