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Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex

The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on oxidative stress parameters induced by aluminium chloride (AlCl(3)) intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h...

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Autores principales: Stevanović, Ivana D., Jovanović, Marina D., Jelenković, Ankica, Čolić, Miodrag, Stojanović, Ivana, Ninković, Milica
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801103/
https://www.ncbi.nlm.nih.gov/pubmed/19255519
http://dx.doi.org/10.4142/jvs.2009.10.1.15
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author Stevanović, Ivana D.
Jovanović, Marina D.
Jelenković, Ankica
Čolić, Miodrag
Stojanović, Ivana
Ninković, Milica
author_facet Stevanović, Ivana D.
Jovanović, Marina D.
Jelenković, Ankica
Čolić, Miodrag
Stojanović, Ivana
Ninković, Milica
author_sort Stevanović, Ivana D.
collection PubMed
description The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on oxidative stress parameters induced by aluminium chloride (AlCl(3)) intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h and 30 d after treatments, heads were immediately frozen in liquid nitrogen and forebrain cortices were removed. Crude mitochondrial fraction preparations of forebrain cortices were used for the biochemical analyses: nitrite levels, superoxide production, malondialdehyde concentrations, superoxide dismutase (SOD) activities and reduced glutathione contents. AlCl(3) injection resulted in increased nitrite concentrations, superoxide anion production, malondialdehyde concentrations and reduced glutathione contents in the forebrain cortex, suggesting that AlCl(3) exposure promoted oxidative stress in this brain structure. The biochemical changes observed in neuronal tissues showed that aluminium acted as a pro-oxidant. However, the non-specific nitric oxide synthase (NOS) inhibitor, L-NAME, exerted anti-oxidant actions in AlCl(3)-treated animals. These results revealed that NO-mediated neurotoxicity due to intrahippocampal AlCl(3) injection spread temporally and spatially to the forebrain cortex, and suggested a potentially neuroprotective effect for L-NAME.
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spelling pubmed-28011032010-01-11 Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex Stevanović, Ivana D. Jovanović, Marina D. Jelenković, Ankica Čolić, Miodrag Stojanović, Ivana Ninković, Milica J Vet Sci Original Article The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on oxidative stress parameters induced by aluminium chloride (AlCl(3)) intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h and 30 d after treatments, heads were immediately frozen in liquid nitrogen and forebrain cortices were removed. Crude mitochondrial fraction preparations of forebrain cortices were used for the biochemical analyses: nitrite levels, superoxide production, malondialdehyde concentrations, superoxide dismutase (SOD) activities and reduced glutathione contents. AlCl(3) injection resulted in increased nitrite concentrations, superoxide anion production, malondialdehyde concentrations and reduced glutathione contents in the forebrain cortex, suggesting that AlCl(3) exposure promoted oxidative stress in this brain structure. The biochemical changes observed in neuronal tissues showed that aluminium acted as a pro-oxidant. However, the non-specific nitric oxide synthase (NOS) inhibitor, L-NAME, exerted anti-oxidant actions in AlCl(3)-treated animals. These results revealed that NO-mediated neurotoxicity due to intrahippocampal AlCl(3) injection spread temporally and spatially to the forebrain cortex, and suggested a potentially neuroprotective effect for L-NAME. The Korean Society of Veterinary Science 2009-03 2009-03-31 /pmc/articles/PMC2801103/ /pubmed/19255519 http://dx.doi.org/10.4142/jvs.2009.10.1.15 Text en Copyright © 2009 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Stevanović, Ivana D.
Jovanović, Marina D.
Jelenković, Ankica
Čolić, Miodrag
Stojanović, Ivana
Ninković, Milica
Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title_full Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title_fullStr Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title_full_unstemmed Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title_short Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl(3)-induced toxicity in the rat forebrain cortex
title_sort effects of l-name, a non-specific nitric oxide synthase inhibitor, on alcl(3)-induced toxicity in the rat forebrain cortex
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801103/
https://www.ncbi.nlm.nih.gov/pubmed/19255519
http://dx.doi.org/10.4142/jvs.2009.10.1.15
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