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A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock

The heat-shock response is characterized by the expression of a set of classical heat-shock genes, and is regulated by heat-shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat...

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Autores principales: Fujimoto, Mitsuaki, Hayashida, Naoki, Katoh, Takuma, Oshima, Kouji, Shinkawa, Toyohide, Prakasam, Ramachandran, Tan, Ke, Inouye, Sachiye, Takii, Ryosuke, Nakai, Akira
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801703/
https://www.ncbi.nlm.nih.gov/pubmed/19864465
http://dx.doi.org/10.1091/mbc.E09-07-0639
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author Fujimoto, Mitsuaki
Hayashida, Naoki
Katoh, Takuma
Oshima, Kouji
Shinkawa, Toyohide
Prakasam, Ramachandran
Tan, Ke
Inouye, Sachiye
Takii, Ryosuke
Nakai, Akira
author_facet Fujimoto, Mitsuaki
Hayashida, Naoki
Katoh, Takuma
Oshima, Kouji
Shinkawa, Toyohide
Prakasam, Ramachandran
Tan, Ke
Inouye, Sachiye
Takii, Ryosuke
Nakai, Akira
author_sort Fujimoto, Mitsuaki
collection PubMed
description The heat-shock response is characterized by the expression of a set of classical heat-shock genes, and is regulated by heat-shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat shock. It is believed that HSF1 is required for the heat-inducible expression of these genes although HSF2 and HSF4 modulate some of the gene expression. Here, we identified a novel mouse HSF3 (mHSF3) translocated into the nucleus during heat shock. However, mHSF3 did not activate classical heat-shock genes such as Hsp70. Remarkably, overexpression of mHSF3 restored the expression of nonclassical heat-shock genes such as PDZK3 and PROM2 in HSF1-null mouse embryonic fibroblasts (MEFs). Although down-regulation of mHSF3 expression had no effect on gene expression or cell survival in wild-type MEF cells, it abolished the moderate expression of PDZK3 mRNA and reduced cell survival in HSF1-null MEF cells during heat shock. We propose that mHSF3 represents a unique HSF that has the potential to activate only nonclassical heat-shock genes to protect cells from detrimental stresses.
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spelling pubmed-28017032010-03-16 A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock Fujimoto, Mitsuaki Hayashida, Naoki Katoh, Takuma Oshima, Kouji Shinkawa, Toyohide Prakasam, Ramachandran Tan, Ke Inouye, Sachiye Takii, Ryosuke Nakai, Akira Mol Biol Cell Articles The heat-shock response is characterized by the expression of a set of classical heat-shock genes, and is regulated by heat-shock transcription factor 1 (HSF1) in mammals. However, comprehensive analyses of gene expression have revealed very large numbers of inducible genes in cells exposed to heat shock. It is believed that HSF1 is required for the heat-inducible expression of these genes although HSF2 and HSF4 modulate some of the gene expression. Here, we identified a novel mouse HSF3 (mHSF3) translocated into the nucleus during heat shock. However, mHSF3 did not activate classical heat-shock genes such as Hsp70. Remarkably, overexpression of mHSF3 restored the expression of nonclassical heat-shock genes such as PDZK3 and PROM2 in HSF1-null mouse embryonic fibroblasts (MEFs). Although down-regulation of mHSF3 expression had no effect on gene expression or cell survival in wild-type MEF cells, it abolished the moderate expression of PDZK3 mRNA and reduced cell survival in HSF1-null MEF cells during heat shock. We propose that mHSF3 represents a unique HSF that has the potential to activate only nonclassical heat-shock genes to protect cells from detrimental stresses. The American Society for Cell Biology 2010-01-01 /pmc/articles/PMC2801703/ /pubmed/19864465 http://dx.doi.org/10.1091/mbc.E09-07-0639 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Fujimoto, Mitsuaki
Hayashida, Naoki
Katoh, Takuma
Oshima, Kouji
Shinkawa, Toyohide
Prakasam, Ramachandran
Tan, Ke
Inouye, Sachiye
Takii, Ryosuke
Nakai, Akira
A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title_full A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title_fullStr A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title_full_unstemmed A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title_short A Novel Mouse HSF3 Has the Potential to Activate Nonclassical Heat-Shock Genes during Heat Shock
title_sort novel mouse hsf3 has the potential to activate nonclassical heat-shock genes during heat shock
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801703/
https://www.ncbi.nlm.nih.gov/pubmed/19864465
http://dx.doi.org/10.1091/mbc.E09-07-0639
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