Cargando…
Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii
We investigated the migration of multiple nuclei in hyphae of the filamentous fungus Ashbya gossypii. Three types of cytoplasmic microtubule (cMT)-dependent nuclear movements were characterized using live cell imaging: short-range oscillations (up to 4.5 μm/min), rotations (up to 180° in 30 s), and...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801712/ https://www.ncbi.nlm.nih.gov/pubmed/19910487 http://dx.doi.org/10.1091/mbc.E09-01-0063 |
_version_ | 1782175955997949952 |
---|---|
author | Lang, Claudia Grava, Sandrine van den Hoorn, Tineke Trimble, Rhonda Philippsen, Peter Jaspersen, Sue L. |
author_facet | Lang, Claudia Grava, Sandrine van den Hoorn, Tineke Trimble, Rhonda Philippsen, Peter Jaspersen, Sue L. |
author_sort | Lang, Claudia |
collection | PubMed |
description | We investigated the migration of multiple nuclei in hyphae of the filamentous fungus Ashbya gossypii. Three types of cytoplasmic microtubule (cMT)-dependent nuclear movements were characterized using live cell imaging: short-range oscillations (up to 4.5 μm/min), rotations (up to 180° in 30 s), and long-range nuclear bypassing (up to 9 μm/min). These movements were superimposed on a cMT-independent mode of nuclear migration, cotransport with the cytoplasmic stream. This latter mode is sufficient to support wild-type-like hyphal growth speeds. cMT-dependent nuclear movements were led by a nuclear-associated microtubule-organizing center, the spindle pole body (SPB), which is the sole site of microtubule nucleation in A. gossypii. Analysis of A. gossypii SPBs by electron microscopy revealed an overall laminar structure similar to the budding yeast SPB but with distinct differences at the cytoplasmic side. Up to six perpendicular and tangential cMTs emanated from a more spherical outer plaque. The perpendicular and tangential cMTs most likely correspond to short, often cortex-associated cMTs and to long, hyphal growth-axis–oriented cMTs, respectively, seen by in vivo imaging. Each SPB nucleates its own array of cMTs, and the lack of overlapping cMT arrays between neighboring nuclei explains the autonomous nuclear oscillations and bypassing observed in A. gossypii hyphae. |
format | Text |
id | pubmed-2801712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28017122010-03-16 Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii Lang, Claudia Grava, Sandrine van den Hoorn, Tineke Trimble, Rhonda Philippsen, Peter Jaspersen, Sue L. Mol Biol Cell Articles We investigated the migration of multiple nuclei in hyphae of the filamentous fungus Ashbya gossypii. Three types of cytoplasmic microtubule (cMT)-dependent nuclear movements were characterized using live cell imaging: short-range oscillations (up to 4.5 μm/min), rotations (up to 180° in 30 s), and long-range nuclear bypassing (up to 9 μm/min). These movements were superimposed on a cMT-independent mode of nuclear migration, cotransport with the cytoplasmic stream. This latter mode is sufficient to support wild-type-like hyphal growth speeds. cMT-dependent nuclear movements were led by a nuclear-associated microtubule-organizing center, the spindle pole body (SPB), which is the sole site of microtubule nucleation in A. gossypii. Analysis of A. gossypii SPBs by electron microscopy revealed an overall laminar structure similar to the budding yeast SPB but with distinct differences at the cytoplasmic side. Up to six perpendicular and tangential cMTs emanated from a more spherical outer plaque. The perpendicular and tangential cMTs most likely correspond to short, often cortex-associated cMTs and to long, hyphal growth-axis–oriented cMTs, respectively, seen by in vivo imaging. Each SPB nucleates its own array of cMTs, and the lack of overlapping cMT arrays between neighboring nuclei explains the autonomous nuclear oscillations and bypassing observed in A. gossypii hyphae. The American Society for Cell Biology 2010-01-01 /pmc/articles/PMC2801712/ /pubmed/19910487 http://dx.doi.org/10.1091/mbc.E09-01-0063 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Lang, Claudia Grava, Sandrine van den Hoorn, Tineke Trimble, Rhonda Philippsen, Peter Jaspersen, Sue L. Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title | Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title_full | Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title_fullStr | Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title_full_unstemmed | Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title_short | Mobility, Microtubule Nucleation and Structure of Microtubule-organizing Centers in Multinucleated Hyphae of Ashbya gossypii |
title_sort | mobility, microtubule nucleation and structure of microtubule-organizing centers in multinucleated hyphae of ashbya gossypii |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801712/ https://www.ncbi.nlm.nih.gov/pubmed/19910487 http://dx.doi.org/10.1091/mbc.E09-01-0063 |
work_keys_str_mv | AT langclaudia mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii AT gravasandrine mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii AT vandenhoorntineke mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii AT trimblerhonda mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii AT philippsenpeter mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii AT jaspersensuel mobilitymicrotubulenucleationandstructureofmicrotubuleorganizingcentersinmultinucleatedhyphaeofashbyagossypii |