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Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation

Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of gro...

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Detalles Bibliográficos
Autores principales: Cortesio, Christa L., Perrin, Benjamin J., Bennin, David A., Huttenlocher, Anna
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801713/
https://www.ncbi.nlm.nih.gov/pubmed/19910490
http://dx.doi.org/10.1091/mbc.E09-02-0106
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author Cortesio, Christa L.
Perrin, Benjamin J.
Bennin, David A.
Huttenlocher, Anna
author_facet Cortesio, Christa L.
Perrin, Benjamin J.
Bennin, David A.
Huttenlocher, Anna
author_sort Cortesio, Christa L.
collection PubMed
description Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor–induced actin-based structures has not been defined. Here we show that mAbp1 localizes to dorsal ruffles and is necessary for platelet-derived growth factor (PDGF)-mediated dorsal ruffle formation. Despite their structural similarity, we find that mAbp1 and cortactin have nonredundant functions in the regulation of dorsal ruffle formation. mAbp1, like cortactin, is a calpain 2 substrate and the preferred cleavage site occurs between the actin-binding domain and the proline-rich region, generating a C-terminal mAbp1 fragment that inhibits dorsal ruffle formation. Furthermore, mAbp1 directly interacts with the actin regulatory protein WASp-interacting protein (WIP) through its SH3 domain. Finally, we demonstrate that the interaction between mAbp1 and WIP is important in regulating dorsal ruffle formation and that WIP-mediated effects on dorsal ruffle formation require mAbp1. Taken together, these findings identify a novel role for mAbp1 in growth factor–induced dorsal ruffle formation through its interaction with WIP.
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spelling pubmed-28017132010-03-16 Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation Cortesio, Christa L. Perrin, Benjamin J. Bennin, David A. Huttenlocher, Anna Mol Biol Cell Articles Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor–induced actin-based structures has not been defined. Here we show that mAbp1 localizes to dorsal ruffles and is necessary for platelet-derived growth factor (PDGF)-mediated dorsal ruffle formation. Despite their structural similarity, we find that mAbp1 and cortactin have nonredundant functions in the regulation of dorsal ruffle formation. mAbp1, like cortactin, is a calpain 2 substrate and the preferred cleavage site occurs between the actin-binding domain and the proline-rich region, generating a C-terminal mAbp1 fragment that inhibits dorsal ruffle formation. Furthermore, mAbp1 directly interacts with the actin regulatory protein WASp-interacting protein (WIP) through its SH3 domain. Finally, we demonstrate that the interaction between mAbp1 and WIP is important in regulating dorsal ruffle formation and that WIP-mediated effects on dorsal ruffle formation require mAbp1. Taken together, these findings identify a novel role for mAbp1 in growth factor–induced dorsal ruffle formation through its interaction with WIP. The American Society for Cell Biology 2010-01-01 /pmc/articles/PMC2801713/ /pubmed/19910490 http://dx.doi.org/10.1091/mbc.E09-02-0106 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Cortesio, Christa L.
Perrin, Benjamin J.
Bennin, David A.
Huttenlocher, Anna
Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title_full Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title_fullStr Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title_full_unstemmed Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title_short Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation
title_sort actin-binding protein-1 interacts with wasp-interacting protein to regulate growth factor-induced dorsal ruffle formation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2801713/
https://www.ncbi.nlm.nih.gov/pubmed/19910490
http://dx.doi.org/10.1091/mbc.E09-02-0106
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AT bennindavida actinbindingprotein1interactswithwaspinteractingproteintoregulategrowthfactorinduceddorsalruffleformation
AT huttenlocheranna actinbindingprotein1interactswithwaspinteractingproteintoregulategrowthfactorinduceddorsalruffleformation