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Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)

PURPOSE: Interleukin (IL)-21 has recently been shown to play a vital role in the development of many autoimmune diseases. Our study is designed to investigate the alteration and possible function of IL-21 in the development of an experimental autoimmune uveitis (EAU) model. METHODS: EAU was induced...

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Autores principales: Liu, Lan, Xu, Yongfeng, Wang, Jianyong, Li, Huiyan
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802297/
https://www.ncbi.nlm.nih.gov/pubmed/20057909
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author Liu, Lan
Xu, Yongfeng
Wang, Jianyong
Li, Huiyan
author_facet Liu, Lan
Xu, Yongfeng
Wang, Jianyong
Li, Huiyan
author_sort Liu, Lan
collection PubMed
description PURPOSE: Interleukin (IL)-21 has recently been shown to play a vital role in the development of many autoimmune diseases. Our study is designed to investigate the alteration and possible function of IL-21 in the development of an experimental autoimmune uveitis (EAU) model. METHODS: EAU was induced in B10.RIII mice by subcutaneous injection of interphotoreceptor retinoid-binding protein (IRBP) 161–180 emulsified with complete Freund’s adjuvant (CFA) and evaluated by clinical and histopathologic observation. IL-21 and IL-21R mRNA expressions in cells of draining lymph node (DLN) and spleen in EAU and control mice were determined by reverse transcription-PCR. The frequencies of interleukin-21 receptor positive cells were also examined using flow cytometry. IL-17 levels in the supernatant of the cell culture upon IL-21 stimulation were assayed by enzyme-linked immunosorbent assay. RESULTS: Results showed that EAU was successfully induced by IRBP161–180. Expression of IL-21 mRNA was significantly increased in cells of DLN and spleen in EAU compared with recovery phase mice and normal controls. IL-21R was also found upregulated in DLN and spleen cells of EAU mice by reverse transcription-PCR and flow cytometry. Cells in EAU cultured with IL-21 combined with transforming growth factor-β induced increased production of IL-17. CONCLUSION: The findings revealed that increased IL-21 and IL-21R expression may be involved in the development of EAU, possibly by promoting IL-17 secretion.
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spelling pubmed-28022972010-01-07 Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU) Liu, Lan Xu, Yongfeng Wang, Jianyong Li, Huiyan Mol Vis Research Article PURPOSE: Interleukin (IL)-21 has recently been shown to play a vital role in the development of many autoimmune diseases. Our study is designed to investigate the alteration and possible function of IL-21 in the development of an experimental autoimmune uveitis (EAU) model. METHODS: EAU was induced in B10.RIII mice by subcutaneous injection of interphotoreceptor retinoid-binding protein (IRBP) 161–180 emulsified with complete Freund’s adjuvant (CFA) and evaluated by clinical and histopathologic observation. IL-21 and IL-21R mRNA expressions in cells of draining lymph node (DLN) and spleen in EAU and control mice were determined by reverse transcription-PCR. The frequencies of interleukin-21 receptor positive cells were also examined using flow cytometry. IL-17 levels in the supernatant of the cell culture upon IL-21 stimulation were assayed by enzyme-linked immunosorbent assay. RESULTS: Results showed that EAU was successfully induced by IRBP161–180. Expression of IL-21 mRNA was significantly increased in cells of DLN and spleen in EAU compared with recovery phase mice and normal controls. IL-21R was also found upregulated in DLN and spleen cells of EAU mice by reverse transcription-PCR and flow cytometry. Cells in EAU cultured with IL-21 combined with transforming growth factor-β induced increased production of IL-17. CONCLUSION: The findings revealed that increased IL-21 and IL-21R expression may be involved in the development of EAU, possibly by promoting IL-17 secretion. Molecular Vision 2009-12-31 /pmc/articles/PMC2802297/ /pubmed/20057909 Text en Copyright © 2008 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Lan
Xu, Yongfeng
Wang, Jianyong
Li, Huiyan
Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title_full Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title_fullStr Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title_full_unstemmed Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title_short Upregulated IL-21 and IL-21 receptor expression is involved in experimental autoimmune uveitis (EAU)
title_sort upregulated il-21 and il-21 receptor expression is involved in experimental autoimmune uveitis (eau)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802297/
https://www.ncbi.nlm.nih.gov/pubmed/20057909
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