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An Agarose-Gel Based Method for Transporting Cell Lines

Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. B...

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Detalles Bibliográficos
Autores principales: Yang, Lingzhi, Li, Chufang, Chen, Ling, Li, Zhiyuan
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802760/
https://www.ncbi.nlm.nih.gov/pubmed/20161836
http://dx.doi.org/10.2174/1875397300903010050
Descripción
Sumario:Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.