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Sulforaphane Increases Cyclin-Dependent Kinase Inhibitor, p21 Protein in Human Oral Carcinoma Cells and Nude Mouse Animal Model to Induce G(2)/M Cell Cycle Arrest

Previously, our group reported that sulforaphane (SFN), a naturally occurring chemopreventive agent from cruciferous vegetables, effectively inhibits the proliferation of KB and YD-10B human oral squamous carcinoma cells by causing apoptosis. In this study, treatment of 20 and 40 µM of SFN for 12 h...

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Detalles Bibliográficos
Autores principales: Kim, Jun-Hee, Han Kwon, Ki, Jung, Ji-Youn, Han, Hye-Suk, Hyun Shim, Jung, Oh, SeJun, Choi, Kyeong-Hee, Choi, Eun-Sun, Shin, Ji-Ae, Leem, Dae-Ho, Soh, Yunjo, Cho, Nam-Pyo, Cho, Sung-Dae
Formato: Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803134/
https://www.ncbi.nlm.nih.gov/pubmed/20104266
http://dx.doi.org/10.3164/jcbn.09-65
Descripción
Sumario:Previously, our group reported that sulforaphane (SFN), a naturally occurring chemopreventive agent from cruciferous vegetables, effectively inhibits the proliferation of KB and YD-10B human oral squamous carcinoma cells by causing apoptosis. In this study, treatment of 20 and 40 µM of SFN for 12 h caused a cell cycle arrest in the G(2)/M phase. Cell cycle arrest induced by SFN was associated with a significant increase in the p21 protein level and a decrease in cyclin B expression, but there was no change in the cyclin A protein level. In addition, SFN increased the p21 promoter activity significantly. Furthermore, SFN induced p21 protein expression in a nude mouse xenograft model suggesting that SFN is a potent inducer of the p21 protein in human oral squamous carcinoma cells. These findings show that SFN is a promising candidate for molecular-targeting chemotherapy against human oral squamous cell carcinoma.