A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment

This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the act...

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Detalles Bibliográficos
Autores principales: Yang, Desuo, Li, Zongxiao, Allan Diwu, Yubo, Fu, Hanzhuo, Liao, Jinfang, Wei, Chunmei, Diwu, Zhenjun
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803437/
https://www.ncbi.nlm.nih.gov/pubmed/20161843
http://dx.doi.org/10.2174/1875397300802010048
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author Yang, Desuo
Li, Zongxiao
Allan Diwu, Yubo
Fu, Hanzhuo
Liao, Jinfang
Wei, Chunmei
Diwu, Zhenjun
author_facet Yang, Desuo
Li, Zongxiao
Allan Diwu, Yubo
Fu, Hanzhuo
Liao, Jinfang
Wei, Chunmei
Diwu, Zhenjun
author_sort Yang, Desuo
collection PubMed
description This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at ~450 nm with low pKa (4.7), high fluorescence quantum yield and pH independence in the physiological pH range. This new fluorescence dye, CF-MU, is a convenient tool for assays with buffer pH between 4.5 and 8.
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spelling pubmed-28034372010-02-16 A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment Yang, Desuo Li, Zongxiao Allan Diwu, Yubo Fu, Hanzhuo Liao, Jinfang Wei, Chunmei Diwu, Zhenjun Curr Chem Genomics Article This article described the synthesis and application of 6-chloro-8-fluoro-4-methylumbelliferone phosphate (CF-MUP) in analyzing acid phosphatase activity. Compared to the existing MUP, the new coumarin phosphate, CF-MUP, demonstrateed much higher sensitivity and was more robust for detecting the activity of acid phosphatase than the classic substrate 4-methylumbelliferone phosphate (MUP). The product of enzyme reaction, 6-chloro-8-fluoro-4-methylumbelliferone (CF-MU) possesses strong fluorescence at ~450 nm with low pKa (4.7), high fluorescence quantum yield and pH independence in the physiological pH range. This new fluorescence dye, CF-MU, is a convenient tool for assays with buffer pH between 4.5 and 8. Bentham Open 2008-11-14 /pmc/articles/PMC2803437/ /pubmed/20161843 http://dx.doi.org/10.2174/1875397300802010048 Text en © Yang et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Yang, Desuo
Li, Zongxiao
Allan Diwu, Yubo
Fu, Hanzhuo
Liao, Jinfang
Wei, Chunmei
Diwu, Zhenjun
A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title_full A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title_fullStr A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title_full_unstemmed A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title_short A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
title_sort novel fluorogenic coumarin substrate for monitoring acid phosphatase activity at low ph environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803437/
https://www.ncbi.nlm.nih.gov/pubmed/20161843
http://dx.doi.org/10.2174/1875397300802010048
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