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Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model

The aim of the present study was to compare the effects of Daivobet(® )and calcipotriol on clinical score and biomarker responses in a modified version of the Scholtz-Dumas psoriasis plaque assay. Furthermore, it was the aim to compare the effects of calcipotriol and betamethasone in the murine psor...

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Autores principales: Kvist, Peter H, Svensson, Lars, Hagberg, Oskar, Hoffmann, Vibeke, Kemp, Kaare, Røpke, Mads A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804591/
https://www.ncbi.nlm.nih.gov/pubmed/20017943
http://dx.doi.org/10.1186/1479-5876-7-107
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author Kvist, Peter H
Svensson, Lars
Hagberg, Oskar
Hoffmann, Vibeke
Kemp, Kaare
Røpke, Mads A
author_facet Kvist, Peter H
Svensson, Lars
Hagberg, Oskar
Hoffmann, Vibeke
Kemp, Kaare
Røpke, Mads A
author_sort Kvist, Peter H
collection PubMed
description The aim of the present study was to compare the effects of Daivobet(® )and calcipotriol on clinical score and biomarker responses in a modified version of the Scholtz-Dumas psoriasis plaque assay. Furthermore, it was the aim to compare the effects of calcipotriol and betamethasone in the murine psoriasis xenograft model. Twenty four patients with psoriasis were treated topically once daily for three weeks, whereas the grafted mice were treated for four weeks. Clinical responses were scored twice weekly and biopsies were taken at the end of each study to analyse for skin biomarkers by histology and immunohistochemistry. The results clearly demonstrate effects on both clinical signs and biomarkers. In the patient study the total clinical score was reduced significantly with both Daivobet(® )and calcipotriol. Both treatments reduced epidermal thickness, Ki-67 and cytokeratin 16 expression. T cell infiltration was significantly reduced by Daivobet(® )but only marginally by calcipotriol. Both treatments showed strong effects on the epidermal psoriatic phenotype. Results from the xenograft model essentially showed the same results. However differences were observed when investigating subtypes of T cells. The study demonstrates the feasibility of obtaining robust biomarker data in the psoriasis plaque test that correlate well with those obtained in other clinical studies. Furthermore, the biomarker data from the plaque test correlate with biopsy data from the grafted mice.
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spelling pubmed-28045912010-01-12 Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model Kvist, Peter H Svensson, Lars Hagberg, Oskar Hoffmann, Vibeke Kemp, Kaare Røpke, Mads A J Transl Med Research The aim of the present study was to compare the effects of Daivobet(® )and calcipotriol on clinical score and biomarker responses in a modified version of the Scholtz-Dumas psoriasis plaque assay. Furthermore, it was the aim to compare the effects of calcipotriol and betamethasone in the murine psoriasis xenograft model. Twenty four patients with psoriasis were treated topically once daily for three weeks, whereas the grafted mice were treated for four weeks. Clinical responses were scored twice weekly and biopsies were taken at the end of each study to analyse for skin biomarkers by histology and immunohistochemistry. The results clearly demonstrate effects on both clinical signs and biomarkers. In the patient study the total clinical score was reduced significantly with both Daivobet(® )and calcipotriol. Both treatments reduced epidermal thickness, Ki-67 and cytokeratin 16 expression. T cell infiltration was significantly reduced by Daivobet(® )but only marginally by calcipotriol. Both treatments showed strong effects on the epidermal psoriatic phenotype. Results from the xenograft model essentially showed the same results. However differences were observed when investigating subtypes of T cells. The study demonstrates the feasibility of obtaining robust biomarker data in the psoriasis plaque test that correlate well with those obtained in other clinical studies. Furthermore, the biomarker data from the plaque test correlate with biopsy data from the grafted mice. BioMed Central 2009-12-17 /pmc/articles/PMC2804591/ /pubmed/20017943 http://dx.doi.org/10.1186/1479-5876-7-107 Text en Copyright ©2009 Kvist et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kvist, Peter H
Svensson, Lars
Hagberg, Oskar
Hoffmann, Vibeke
Kemp, Kaare
Røpke, Mads A
Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title_full Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title_fullStr Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title_full_unstemmed Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title_short Comparison of the effects of vitamin D products in a psoriasis plaque test and a murine psoriasis xenograft model
title_sort comparison of the effects of vitamin d products in a psoriasis plaque test and a murine psoriasis xenograft model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804591/
https://www.ncbi.nlm.nih.gov/pubmed/20017943
http://dx.doi.org/10.1186/1479-5876-7-107
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