Cargando…
Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium
BACKGROUND: To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube. METHODS: The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube t...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2805671/ https://www.ncbi.nlm.nih.gov/pubmed/20040080 http://dx.doi.org/10.1186/1477-7827-7-152 |
_version_ | 1782176215640047616 |
---|---|
author | Amir, Michal Romano, Shabtai Goldman, Shlomit Shalev, Eliezer |
author_facet | Amir, Michal Romano, Shabtai Goldman, Shlomit Shalev, Eliezer |
author_sort | Amir, Michal |
collection | PubMed |
description | BACKGROUND: To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube. METHODS: The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted using a total RNA isolation kit. Semi-quantitative RT-PCR was performed to examine mRNA relative expression. RESULTS: Plexin-B1 expression in the endometrium was significantly higher on days 19 - 23 compared to days 12 - 14 (1.166 +/- 0.42 versus 0.523 +/- 0.299), P < 0.005. In the fallopian tube the level of plexin-B1 did not change significantly throughout the menstrual cycle. Glycodelin expression was significantly higher on days 19 - 23 compared with days 12-14, both in the endometrium (0.819 +/- 0.564 versus 0.072 +/- 0.343, P < 0.05) and the fallopian tube (0.796 +/- 0.196 versus 0.329 +/- 0.398, P < 0.05). Although the level of MMP7 secretion was the highest in the secretory phase the difference from the proliferative phase did not reach statistical significance, neither in the endometrium nor in the fallopian tube. This could result from a lack of power. CONCLUSIONS: In the endometrium, both Glycodelin and Plexin-B1 are exhibiting a cyclic pattern suggesting a possible steroid regulation and a role in endometrial receptivity. |
format | Text |
id | pubmed-2805671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28056712010-01-13 Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium Amir, Michal Romano, Shabtai Goldman, Shlomit Shalev, Eliezer Reprod Biol Endocrinol Research BACKGROUND: To study the expression of Plexin-B1, Glycodelin, and MMP7 during the menstrual cycle in the endometrium and in the fallopian tube. METHODS: The research included women undergoing hysterectomy, tubal sterilization or salpingo-oophoerectomy. Total RNA from endometrial and fallopian tube tissues was extracted using a total RNA isolation kit. Semi-quantitative RT-PCR was performed to examine mRNA relative expression. RESULTS: Plexin-B1 expression in the endometrium was significantly higher on days 19 - 23 compared to days 12 - 14 (1.166 +/- 0.42 versus 0.523 +/- 0.299), P < 0.005. In the fallopian tube the level of plexin-B1 did not change significantly throughout the menstrual cycle. Glycodelin expression was significantly higher on days 19 - 23 compared with days 12-14, both in the endometrium (0.819 +/- 0.564 versus 0.072 +/- 0.343, P < 0.05) and the fallopian tube (0.796 +/- 0.196 versus 0.329 +/- 0.398, P < 0.05). Although the level of MMP7 secretion was the highest in the secretory phase the difference from the proliferative phase did not reach statistical significance, neither in the endometrium nor in the fallopian tube. This could result from a lack of power. CONCLUSIONS: In the endometrium, both Glycodelin and Plexin-B1 are exhibiting a cyclic pattern suggesting a possible steroid regulation and a role in endometrial receptivity. BioMed Central 2009-12-29 /pmc/articles/PMC2805671/ /pubmed/20040080 http://dx.doi.org/10.1186/1477-7827-7-152 Text en Copyright ©2009 Amir et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Amir, Michal Romano, Shabtai Goldman, Shlomit Shalev, Eliezer Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title | Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title_full | Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title_fullStr | Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title_full_unstemmed | Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title_short | Plexin-B1, glycodelin and MMP7 expression in the human fallopian tube and in the endometrium |
title_sort | plexin-b1, glycodelin and mmp7 expression in the human fallopian tube and in the endometrium |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2805671/ https://www.ncbi.nlm.nih.gov/pubmed/20040080 http://dx.doi.org/10.1186/1477-7827-7-152 |
work_keys_str_mv | AT amirmichal plexinb1glycodelinandmmp7expressioninthehumanfallopiantubeandintheendometrium AT romanoshabtai plexinb1glycodelinandmmp7expressioninthehumanfallopiantubeandintheendometrium AT goldmanshlomit plexinb1glycodelinandmmp7expressioninthehumanfallopiantubeandintheendometrium AT shaleveliezer plexinb1glycodelinandmmp7expressioninthehumanfallopiantubeandintheendometrium |