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Altered Levels of Acetylcholinesterase in Alzheimer Plasma
BACKGROUND: Many studies have been conducted in an extensive effort to identify alterations in blood cholinesterase levels as a consequence of disease, including the analysis of acetylcholinesterase (AChE) in plasma. Conventional assays using selective cholinesterase inhibitors have not been particu...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806824/ https://www.ncbi.nlm.nih.gov/pubmed/20090844 http://dx.doi.org/10.1371/journal.pone.0008701 |
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author | García-Ayllón, María-Salud Riba-Llena, Iolanda Serra-Basante, Carol Alom, Jordi Boopathy, Rathnam Sáez-Valero, Javier |
author_facet | García-Ayllón, María-Salud Riba-Llena, Iolanda Serra-Basante, Carol Alom, Jordi Boopathy, Rathnam Sáez-Valero, Javier |
author_sort | García-Ayllón, María-Salud |
collection | PubMed |
description | BACKGROUND: Many studies have been conducted in an extensive effort to identify alterations in blood cholinesterase levels as a consequence of disease, including the analysis of acetylcholinesterase (AChE) in plasma. Conventional assays using selective cholinesterase inhibitors have not been particularly successful as excess amounts of butyrylcholinesterase (BuChE) pose a major problem. PRINCIPAL FINDINGS: Here we have estimated the levels of AChE activity in human plasma by first immunoprecipitating BuChE and measuring AChE activity in the immunodepleted plasma. Human plasma AChE activity levels were ∼20 nmol/min/mL, about 160 times lower than BuChE. The majority of AChE species are the light G(1)+G(2) forms and not G(4) tetramers. The levels and pattern of the molecular forms are similar to that observed in individuals with silent BuChE. We have also compared plasma AChE with the enzyme pattern obtained from human liver, red blood cells, cerebrospinal fluid (CSF) and brain, by sedimentation analysis, Western blotting and lectin-binding analysis. Finally, a selective increase of AChE activity was detected in plasma from Alzheimer's disease (AD) patients compared to age and gender-matched controls. This increase correlates with an increase in the G(1)+G(2) forms, the subset of AChE species which are increased in Alzheimer's brain. Western blot analysis demonstrated that a 78 kDa immunoreactive AChE protein band was also increased in Alzheimer's plasma, attributed in part to AChE-T subunits common in brain and CSF. CONCLUSION: Plasma AChE might have potential as an indicator of disease progress and prognosis in AD and warrants further investigation. |
format | Text |
id | pubmed-2806824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28068242010-01-20 Altered Levels of Acetylcholinesterase in Alzheimer Plasma García-Ayllón, María-Salud Riba-Llena, Iolanda Serra-Basante, Carol Alom, Jordi Boopathy, Rathnam Sáez-Valero, Javier PLoS One Research Article BACKGROUND: Many studies have been conducted in an extensive effort to identify alterations in blood cholinesterase levels as a consequence of disease, including the analysis of acetylcholinesterase (AChE) in plasma. Conventional assays using selective cholinesterase inhibitors have not been particularly successful as excess amounts of butyrylcholinesterase (BuChE) pose a major problem. PRINCIPAL FINDINGS: Here we have estimated the levels of AChE activity in human plasma by first immunoprecipitating BuChE and measuring AChE activity in the immunodepleted plasma. Human plasma AChE activity levels were ∼20 nmol/min/mL, about 160 times lower than BuChE. The majority of AChE species are the light G(1)+G(2) forms and not G(4) tetramers. The levels and pattern of the molecular forms are similar to that observed in individuals with silent BuChE. We have also compared plasma AChE with the enzyme pattern obtained from human liver, red blood cells, cerebrospinal fluid (CSF) and brain, by sedimentation analysis, Western blotting and lectin-binding analysis. Finally, a selective increase of AChE activity was detected in plasma from Alzheimer's disease (AD) patients compared to age and gender-matched controls. This increase correlates with an increase in the G(1)+G(2) forms, the subset of AChE species which are increased in Alzheimer's brain. Western blot analysis demonstrated that a 78 kDa immunoreactive AChE protein band was also increased in Alzheimer's plasma, attributed in part to AChE-T subunits common in brain and CSF. CONCLUSION: Plasma AChE might have potential as an indicator of disease progress and prognosis in AD and warrants further investigation. Public Library of Science 2010-01-14 /pmc/articles/PMC2806824/ /pubmed/20090844 http://dx.doi.org/10.1371/journal.pone.0008701 Text en García-Ayllón et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article García-Ayllón, María-Salud Riba-Llena, Iolanda Serra-Basante, Carol Alom, Jordi Boopathy, Rathnam Sáez-Valero, Javier Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title | Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title_full | Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title_fullStr | Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title_full_unstemmed | Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title_short | Altered Levels of Acetylcholinesterase in Alzheimer Plasma |
title_sort | altered levels of acetylcholinesterase in alzheimer plasma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806824/ https://www.ncbi.nlm.nih.gov/pubmed/20090844 http://dx.doi.org/10.1371/journal.pone.0008701 |
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