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Human CD4(+) Memory T Cells Can Become CD4(+)IL-9(+) T Cells
BACKGROUND: IL-9 is a growth factor for T- and mast-cells that is secreted by human Th2 cells. We recently reported that IL-4+TGF-β directs mouse CD4(+)CD25(−)CD62L(+) T cells to commit to inflammatory IL-9 producing CD4(+) T cells. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that human inducible r...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806834/ https://www.ncbi.nlm.nih.gov/pubmed/20090929 http://dx.doi.org/10.1371/journal.pone.0008706 |
Sumario: | BACKGROUND: IL-9 is a growth factor for T- and mast-cells that is secreted by human Th2 cells. We recently reported that IL-4+TGF-β directs mouse CD4(+)CD25(−)CD62L(+) T cells to commit to inflammatory IL-9 producing CD4(+) T cells. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that human inducible regulatory T cells (iTregs) also express IL-9. IL-4+TGF-β induced higher levels of IL-9 expression in plate bound-anti-CD3 mAb (pbCD3)/soluble-anti-CD28 mAb (sCD28) activated human resting memory CD4(+)CD25(−)CD45RO(+) T cells as compared to naïve CD4(+)CD25(−)CD45RA(+) T cells. In addition, as compared to pbCD3/sCD28 plus TGF-β stimulation, IL-4+TGF-β stimulated memory CD4(+)CD25(−)CD45RO(+) T cells expressed reduced FOXP3 protein. As analyzed by pre-amplification boosted single-cell real-time PCR, human CD4(+)IL-9(+) T cells expressed GATA3 and RORC, but not IL-10, IL-13, IFNγ or IL-17A/F. Attempts to optimize IL-9 production by pbCD3/sCD28 and IL-4+TGF-β stimulated resting memory CD4(+) T cells demonstrated that the addition of IL-1β, IL-12, and IL-21 further enhance IL-9 production. CONCLUSIONS/SIGNIFICANCE: Taken together these data show both the differences and similarities between mouse and human CD4(+)IL9(+) T cells and reaffirm the powerful influence of inflammatory cytokines to shape the response of activated CD4(+) T cells to antigen. |
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