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Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients
Stroke is a major cause of disability and leading cause of death in the northern hemisphere. Only recently it became evident that cerebral ischemia not only leads to brain tissue damage and subsequent local inflammation but also to a dramatic loss of peripheral blood T-cells with subsequent infectio...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806837/ https://www.ncbi.nlm.nih.gov/pubmed/20090932 http://dx.doi.org/10.1371/journal.pone.0008718 |
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author | Vogelgesang, Antje May, Verena E. L. Grunwald, Uwe Bakkeboe, Maren Langner, Soenke Wallaschofski, Henry Kessler, Christof Bröker, Barbara M. Dressel, Alexander |
author_facet | Vogelgesang, Antje May, Verena E. L. Grunwald, Uwe Bakkeboe, Maren Langner, Soenke Wallaschofski, Henry Kessler, Christof Bröker, Barbara M. Dressel, Alexander |
author_sort | Vogelgesang, Antje |
collection | PubMed |
description | Stroke is a major cause of disability and leading cause of death in the northern hemisphere. Only recently it became evident that cerebral ischemia not only leads to brain tissue damage and subsequent local inflammation but also to a dramatic loss of peripheral blood T-cells with subsequent infections. However, only scarce information is available on the activation status of surviving T cells. This study therefore addressed the functional consequences of immunological changes induced by stroke in humans. For this purpose peripheral blood T-cells were isolated from 93 stroke patients and the expression of activation makers was determined. In addition ex vivo stimulation assays were applied to asses the functionality of T cells derived from blood of stroke patients. Compared to healthy controls, stroke patients demonstrated an enhanced surface expression of HLA-DR (p<0.0001) and CD25 (p = 0.02) on T cells, revealing that stroke leads to T cell activation, while CTLA-4 remained undetectable. In vitro studies revealed that catecholamines inhibit CTLA-4 upregulation in activated T cells. Ex vivo, T cells of stroke patients proliferated unimpaired and released increased amounts of the proinflammatory cytokine TNF-α (p<0.01) and IL-6 (p<0.05). Also, in sera of stroke patients HMGB1 concentrations were increased (p = 0.0002). The data demonstrate that surviving T cells in stroke patients remain fully functional and are primed towards a TH1 response, in addition we provide evidence that catecholamine mediated inhibition of CTLA-4 expression and serum HMGB1 release are possible mediators in stroke induced activation of T cells. |
format | Text |
id | pubmed-2806837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28068372010-01-20 Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients Vogelgesang, Antje May, Verena E. L. Grunwald, Uwe Bakkeboe, Maren Langner, Soenke Wallaschofski, Henry Kessler, Christof Bröker, Barbara M. Dressel, Alexander PLoS One Research Article Stroke is a major cause of disability and leading cause of death in the northern hemisphere. Only recently it became evident that cerebral ischemia not only leads to brain tissue damage and subsequent local inflammation but also to a dramatic loss of peripheral blood T-cells with subsequent infections. However, only scarce information is available on the activation status of surviving T cells. This study therefore addressed the functional consequences of immunological changes induced by stroke in humans. For this purpose peripheral blood T-cells were isolated from 93 stroke patients and the expression of activation makers was determined. In addition ex vivo stimulation assays were applied to asses the functionality of T cells derived from blood of stroke patients. Compared to healthy controls, stroke patients demonstrated an enhanced surface expression of HLA-DR (p<0.0001) and CD25 (p = 0.02) on T cells, revealing that stroke leads to T cell activation, while CTLA-4 remained undetectable. In vitro studies revealed that catecholamines inhibit CTLA-4 upregulation in activated T cells. Ex vivo, T cells of stroke patients proliferated unimpaired and released increased amounts of the proinflammatory cytokine TNF-α (p<0.01) and IL-6 (p<0.05). Also, in sera of stroke patients HMGB1 concentrations were increased (p = 0.0002). The data demonstrate that surviving T cells in stroke patients remain fully functional and are primed towards a TH1 response, in addition we provide evidence that catecholamine mediated inhibition of CTLA-4 expression and serum HMGB1 release are possible mediators in stroke induced activation of T cells. Public Library of Science 2010-01-14 /pmc/articles/PMC2806837/ /pubmed/20090932 http://dx.doi.org/10.1371/journal.pone.0008718 Text en Vogelgesang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Vogelgesang, Antje May, Verena E. L. Grunwald, Uwe Bakkeboe, Maren Langner, Soenke Wallaschofski, Henry Kessler, Christof Bröker, Barbara M. Dressel, Alexander Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title | Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title_full | Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title_fullStr | Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title_full_unstemmed | Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title_short | Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients |
title_sort | functional status of peripheral blood t-cells in ischemic stroke patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806837/ https://www.ncbi.nlm.nih.gov/pubmed/20090932 http://dx.doi.org/10.1371/journal.pone.0008718 |
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