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Transposon Assisted Gene Insertion Technology (TAGIT): A Tool for Generating Fluorescent Fusion Proteins

We constructed a transposon (transposon assisted gene insertion technology, or TAGIT) that allows the random insertion of gfp (or other genes) into chromosomal loci without disrupting operon structure or regulation. TAGIT is a modified Tn5 transposon that uses Kan(R) to select for insertions on the...

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Detalles Bibliográficos
Autores principales:	Gregory, James A., Becker, Eric C., Jung, James, Tuwatananurak, Ida, Pogliano, Kit
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2806921/ https://www.ncbi.nlm.nih.gov/pubmed/20090956 http://dx.doi.org/10.1371/journal.pone.0008731

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