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Nuclear transfer: Progress and quandaries

Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Amo...

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Detalles Bibliográficos
Autores principales: Li, Xuemei, Li, Ziyi, Jouneau, Alice, Zhou, Qi, Renard, Jean-Paul
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC280728/
https://www.ncbi.nlm.nih.gov/pubmed/14613540
http://dx.doi.org/10.1186/1477-7827-1-84
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author Li, Xuemei
Li, Ziyi
Jouneau, Alice
Zhou, Qi
Renard, Jean-Paul
author_facet Li, Xuemei
Li, Ziyi
Jouneau, Alice
Zhou, Qi
Renard, Jean-Paul
author_sort Li, Xuemei
collection PubMed
description Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles include an incomplete understanding of biologic processes that control epigenetic reprogramming of the donor genome following nuclear transfer. Incomplete epigenetic reprogramming is considered the major cause of the developmental failure of cloned embryos and is frequently associated with the disregulation of specific genes. At present, little is known about the developmental mechanism of reconstructed embryos. Therefore, screening strategies to design nuclear transfer protocols that will mimic the epigenetic remodeling occurring in normal embryos and identifying molecular parameters that can assess the developmental potential of pre-implantation embryos are becoming increasingly important. A crucial need at present is to understand the molecular events required for efficient reprogramming of donor genomes after nuclear transfer. This knowledge will help to identify the molecular basis of developmental defects seen in cloned embryos and provide methods for circumventing such problems associated with cloning the future application of this technology.
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spelling pubmed-2807282003-12-02 Nuclear transfer: Progress and quandaries Li, Xuemei Li, Ziyi Jouneau, Alice Zhou, Qi Renard, Jean-Paul Reprod Biol Endocrinol Review Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles include an incomplete understanding of biologic processes that control epigenetic reprogramming of the donor genome following nuclear transfer. Incomplete epigenetic reprogramming is considered the major cause of the developmental failure of cloned embryos and is frequently associated with the disregulation of specific genes. At present, little is known about the developmental mechanism of reconstructed embryos. Therefore, screening strategies to design nuclear transfer protocols that will mimic the epigenetic remodeling occurring in normal embryos and identifying molecular parameters that can assess the developmental potential of pre-implantation embryos are becoming increasingly important. A crucial need at present is to understand the molecular events required for efficient reprogramming of donor genomes after nuclear transfer. This knowledge will help to identify the molecular basis of developmental defects seen in cloned embryos and provide methods for circumventing such problems associated with cloning the future application of this technology. BioMed Central 2003-11-07 /pmc/articles/PMC280728/ /pubmed/14613540 http://dx.doi.org/10.1186/1477-7827-1-84 Text en Copyright © 2003 Li et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Review
Li, Xuemei
Li, Ziyi
Jouneau, Alice
Zhou, Qi
Renard, Jean-Paul
Nuclear transfer: Progress and quandaries
title Nuclear transfer: Progress and quandaries
title_full Nuclear transfer: Progress and quandaries
title_fullStr Nuclear transfer: Progress and quandaries
title_full_unstemmed Nuclear transfer: Progress and quandaries
title_short Nuclear transfer: Progress and quandaries
title_sort nuclear transfer: progress and quandaries
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC280728/
https://www.ncbi.nlm.nih.gov/pubmed/14613540
http://dx.doi.org/10.1186/1477-7827-1-84
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