Cargando…
Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optic...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807596/ https://www.ncbi.nlm.nih.gov/pubmed/19756838 http://dx.doi.org/10.1007/s10103-009-0723-y |
_version_ | 1782176414177427456 |
---|---|
author | van der Meer, Freek J. Faber, Dirk J. Aalders, Maurice C. G. Poot, Andre A. Vermes, Istvan van Leeuwen, Ton G. |
author_facet | van der Meer, Freek J. Faber, Dirk J. Aalders, Maurice C. G. Poot, Andre A. Vermes, Istvan van Leeuwen, Ton G. |
author_sort | van der Meer, Freek J. |
collection | PubMed |
description | Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm(−1) to 1.3 ± 0.6 mm(−1), whereas, in the apoptotic cells, an increase to 6.4 ± 1.7 mm(−1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment. |
format | Text |
id | pubmed-2807596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-28075962010-01-22 Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography van der Meer, Freek J. Faber, Dirk J. Aalders, Maurice C. G. Poot, Andre A. Vermes, Istvan van Leeuwen, Ton G. Lasers Med Sci Original Article Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm(−1) to 1.3 ± 0.6 mm(−1), whereas, in the apoptotic cells, an increase to 6.4 ± 1.7 mm(−1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment. Springer-Verlag 2009-09-10 2010 /pmc/articles/PMC2807596/ /pubmed/19756838 http://dx.doi.org/10.1007/s10103-009-0723-y Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Article van der Meer, Freek J. Faber, Dirk J. Aalders, Maurice C. G. Poot, Andre A. Vermes, Istvan van Leeuwen, Ton G. Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title | Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title_full | Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title_fullStr | Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title_full_unstemmed | Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title_short | Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
title_sort | apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807596/ https://www.ncbi.nlm.nih.gov/pubmed/19756838 http://dx.doi.org/10.1007/s10103-009-0723-y |
work_keys_str_mv | AT vandermeerfreekj apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography AT faberdirkj apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography AT aaldersmauricecg apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography AT pootandrea apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography AT vermesistvan apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography AT vanleeuwentong apoptosisandnecrosisinducedchangesinlightattenuationmeasuredbyopticalcoherencetomography |