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Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography

Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optic...

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Autores principales: van der Meer, Freek J., Faber, Dirk J., Aalders, Maurice C. G., Poot, Andre A., Vermes, Istvan, van Leeuwen, Ton G.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807596/
https://www.ncbi.nlm.nih.gov/pubmed/19756838
http://dx.doi.org/10.1007/s10103-009-0723-y
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author van der Meer, Freek J.
Faber, Dirk J.
Aalders, Maurice C. G.
Poot, Andre A.
Vermes, Istvan
van Leeuwen, Ton G.
author_facet van der Meer, Freek J.
Faber, Dirk J.
Aalders, Maurice C. G.
Poot, Andre A.
Vermes, Istvan
van Leeuwen, Ton G.
author_sort van der Meer, Freek J.
collection PubMed
description Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm(−1) to 1.3 ± 0.6 mm(−1), whereas, in the apoptotic cells, an increase to 6.4 ± 1.7 mm(−1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment.
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spelling pubmed-28075962010-01-22 Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography van der Meer, Freek J. Faber, Dirk J. Aalders, Maurice C. G. Poot, Andre A. Vermes, Istvan van Leeuwen, Ton G. Lasers Med Sci Original Article Optical coherence tomography (OCT) was used to determine optical properties of pelleted human fibroblasts in which necrosis or apoptosis had been induced. We analysed the OCT data, including both the scattering properties of the medium and the axial point spread function of the OCT system. The optical attenuation coefficient in necrotic cells decreased from 2.2 ± 0.3 mm(−1) to 1.3 ± 0.6 mm(−1), whereas, in the apoptotic cells, an increase to 6.4 ± 1.7 mm(−1) was observed. The results from cultured cells, as presented in this study, indicate the ability of OCT to detect and differentiate between viable, apoptotic, and necrotic cells, based on their attenuation coefficient. This functional supplement to high-resolution OCT imaging can be of great clinical benefit, enabling on-line monitoring of tissues, e.g. for feedback in cancer treatment. Springer-Verlag 2009-09-10 2010 /pmc/articles/PMC2807596/ /pubmed/19756838 http://dx.doi.org/10.1007/s10103-009-0723-y Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
van der Meer, Freek J.
Faber, Dirk J.
Aalders, Maurice C. G.
Poot, Andre A.
Vermes, Istvan
van Leeuwen, Ton G.
Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title_full Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title_fullStr Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title_full_unstemmed Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title_short Apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
title_sort apoptosis- and necrosis-induced changes in light attenuation measured by optical coherence tomography
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807596/
https://www.ncbi.nlm.nih.gov/pubmed/19756838
http://dx.doi.org/10.1007/s10103-009-0723-y
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