Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags

PURPOSE: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. METHODS: mR...

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Autores principales: Maki, Nobuyasu, Martinson, John, Nishimura, Osamu, Tarui, Hiroshi, Meller, Jaroslaw, Tsonis, Panagiotis A., Agata, Kiyokazu
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807617/
https://www.ncbi.nlm.nih.gov/pubmed/20090923
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author Maki, Nobuyasu
Martinson, John
Nishimura, Osamu
Tarui, Hiroshi
Meller, Jaroslaw
Tsonis, Panagiotis A.
Agata, Kiyokazu
author_facet Maki, Nobuyasu
Martinson, John
Nishimura, Osamu
Tarui, Hiroshi
Meller, Jaroslaw
Tsonis, Panagiotis A.
Agata, Kiyokazu
author_sort Maki, Nobuyasu
collection PubMed
description PURPOSE: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. METHODS: mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed. RESULTS: From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found. CONCLUSIONS: The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).
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spelling pubmed-28076172010-01-20 Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags Maki, Nobuyasu Martinson, John Nishimura, Osamu Tarui, Hiroshi Meller, Jaroslaw Tsonis, Panagiotis A. Agata, Kiyokazu Mol Vis Research Article PURPOSE: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. METHODS: mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed. RESULTS: From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosis-related genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found. CONCLUSIONS: The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559). Molecular Vision 2010-01-18 /pmc/articles/PMC2807617/ /pubmed/20090923 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Maki, Nobuyasu
Martinson, John
Nishimura, Osamu
Tarui, Hiroshi
Meller, Jaroslaw
Tsonis, Panagiotis A.
Agata, Kiyokazu
Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title_full Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title_fullStr Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title_full_unstemmed Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title_short Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
title_sort expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807617/
https://www.ncbi.nlm.nih.gov/pubmed/20090923
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