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Developmental dynamics of cone photoreceptors in the eel

BACKGROUND: Many fish alter their expressed visual pigments during development. The number of retinal opsins expressed and their type is normally related to the environment in which they live. Eels are known to change the expression of their rod opsins as they mature, but might they also change the...

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Autores principales: Cottrill, Phillippa B, Davies, Wayne L, Semo, Ma'ayan, Bowmaker, James K, Hunt, David M, Jeffery, Glen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807862/
https://www.ncbi.nlm.nih.gov/pubmed/20025774
http://dx.doi.org/10.1186/1471-213X-9-71
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author Cottrill, Phillippa B
Davies, Wayne L
Semo, Ma'ayan
Bowmaker, James K
Hunt, David M
Jeffery, Glen
author_facet Cottrill, Phillippa B
Davies, Wayne L
Semo, Ma'ayan
Bowmaker, James K
Hunt, David M
Jeffery, Glen
author_sort Cottrill, Phillippa B
collection PubMed
description BACKGROUND: Many fish alter their expressed visual pigments during development. The number of retinal opsins expressed and their type is normally related to the environment in which they live. Eels are known to change the expression of their rod opsins as they mature, but might they also change the expression of their cone opsins? RESULTS: The Rh2 and Sws2 opsin sequences from the European Eel were isolated, sequenced and expressed in vitro for an accurate measurement of their λ(max )values. In situ hybridisation revealed that glass eels express only rh2 opsin in their cone photoreceptors, while larger yellow eels continue to express rh2 opsin in the majority of their cones, but also have <5% of cones which express sws2 opsin. Silver eels showed the same expression pattern as the larger yellow eels. This observation was confirmed by qPCR (quantitative polymerase chain reaction). CONCLUSIONS: Larger yellow and silver European eels express two different cone opsins, rh2 and sws2. This work demonstrates that only the Rh2 cone opsin is present in younger fish (smaller yellow and glass), the sws2 opsin being expressed additionally only by older fish and only in <5% of cone cells.
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spelling pubmed-28078622010-01-19 Developmental dynamics of cone photoreceptors in the eel Cottrill, Phillippa B Davies, Wayne L Semo, Ma'ayan Bowmaker, James K Hunt, David M Jeffery, Glen BMC Dev Biol Research article BACKGROUND: Many fish alter their expressed visual pigments during development. The number of retinal opsins expressed and their type is normally related to the environment in which they live. Eels are known to change the expression of their rod opsins as they mature, but might they also change the expression of their cone opsins? RESULTS: The Rh2 and Sws2 opsin sequences from the European Eel were isolated, sequenced and expressed in vitro for an accurate measurement of their λ(max )values. In situ hybridisation revealed that glass eels express only rh2 opsin in their cone photoreceptors, while larger yellow eels continue to express rh2 opsin in the majority of their cones, but also have <5% of cones which express sws2 opsin. Silver eels showed the same expression pattern as the larger yellow eels. This observation was confirmed by qPCR (quantitative polymerase chain reaction). CONCLUSIONS: Larger yellow and silver European eels express two different cone opsins, rh2 and sws2. This work demonstrates that only the Rh2 cone opsin is present in younger fish (smaller yellow and glass), the sws2 opsin being expressed additionally only by older fish and only in <5% of cone cells. BioMed Central 2009-12-21 /pmc/articles/PMC2807862/ /pubmed/20025774 http://dx.doi.org/10.1186/1471-213X-9-71 Text en Copyright ©2009 Cottrill et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Cottrill, Phillippa B
Davies, Wayne L
Semo, Ma'ayan
Bowmaker, James K
Hunt, David M
Jeffery, Glen
Developmental dynamics of cone photoreceptors in the eel
title Developmental dynamics of cone photoreceptors in the eel
title_full Developmental dynamics of cone photoreceptors in the eel
title_fullStr Developmental dynamics of cone photoreceptors in the eel
title_full_unstemmed Developmental dynamics of cone photoreceptors in the eel
title_short Developmental dynamics of cone photoreceptors in the eel
title_sort developmental dynamics of cone photoreceptors in the eel
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807862/
https://www.ncbi.nlm.nih.gov/pubmed/20025774
http://dx.doi.org/10.1186/1471-213X-9-71
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