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On Optical Detection of Densely Labeled Synapses in Neuropil and Mapping Connectivity with Combinatorially Multiplexed Fluorescent Synaptic Markers
We propose a new method for mapping neural connectivity optically, by utilizing Cre/Lox system Brainbow to tag synapses of different neurons with random mixtures of different fluorophores, such as GFP, YFP, etc., and then detecting patterns of fluorophores at different synapses using light microscop...
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| Formato: | Texto |
| Lenguaje: | English |
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Public Library of Science
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809746/ https://www.ncbi.nlm.nih.gov/pubmed/20107507 http://dx.doi.org/10.1371/journal.pone.0008853 |