Cargando…
HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810064/ https://www.ncbi.nlm.nih.gov/pubmed/20177471 http://dx.doi.org/10.4103/0250-474X.51951 |
_version_ | 1782176658378194944 |
---|---|
author | Joshi, Maithilee Nikalje, A. P. Shahed, M. Dehghan, M. |
author_facet | Joshi, Maithilee Nikalje, A. P. Shahed, M. Dehghan, M. |
author_sort | Joshi, Maithilee |
collection | PubMed |
description | A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995. The proposed method can be successfully used to determine the drug content of marketed tablet formulation. |
format | Text |
id | pubmed-2810064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-28100642010-02-22 HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form Joshi, Maithilee Nikalje, A. P. Shahed, M. Dehghan, M. Indian J Pharm Sci Short Communication A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of emtricitabine and tenofovir simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F 254. The mobile phase used was a mixture of chloroform: methanol (9:1 v/v). The detection of spots was carried out at 265 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng with regression coefficient of 0.9995. The proposed method can be successfully used to determine the drug content of marketed tablet formulation. Medknow Publications 2009 /pmc/articles/PMC2810064/ /pubmed/20177471 http://dx.doi.org/10.4103/0250-474X.51951 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Joshi, Maithilee Nikalje, A. P. Shahed, M. Dehghan, M. HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title | HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title_full | HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title_fullStr | HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title_full_unstemmed | HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title_short | HPTLC Method for the Simultaneous Estimation of Emtricitabine and Tenofovir in Tablet Dosage Form |
title_sort | hptlc method for the simultaneous estimation of emtricitabine and tenofovir in tablet dosage form |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810064/ https://www.ncbi.nlm.nih.gov/pubmed/20177471 http://dx.doi.org/10.4103/0250-474X.51951 |
work_keys_str_mv | AT joshimaithilee hptlcmethodforthesimultaneousestimationofemtricitabineandtenofovirintabletdosageform AT nikaljeap hptlcmethodforthesimultaneousestimationofemtricitabineandtenofovirintabletdosageform AT shahedm hptlcmethodforthesimultaneousestimationofemtricitabineandtenofovirintabletdosageform AT dehghanm hptlcmethodforthesimultaneousestimationofemtricitabineandtenofovirintabletdosageform |