Cargando…
Organization of membrane motor in outer hair cells: an atomic force microscopic study
Using atomic force microscopy, we imaged the cytosolic surface of the lateral plasma membrane of outer hair cells from guinea pigs’ inner ear. We used a “cell-free” preparation, in which a patch of plasma membrane was firmly attached to a substrate and the cytoplasmic face was exposed. The membrane...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810367/ https://www.ncbi.nlm.nih.gov/pubmed/19809831 http://dx.doi.org/10.1007/s00424-009-0742-3 |
_version_ | 1782176678746783744 |
---|---|
author | Sinha, Ghanshyam P. Sabri, Firouzeh Dimitriadis, Emilios K. Iwasa, Kuni H. |
author_facet | Sinha, Ghanshyam P. Sabri, Firouzeh Dimitriadis, Emilios K. Iwasa, Kuni H. |
author_sort | Sinha, Ghanshyam P. |
collection | PubMed |
description | Using atomic force microscopy, we imaged the cytosolic surface of the lateral plasma membrane of outer hair cells from guinea pigs’ inner ear. We used a “cell-free” preparation, in which a patch of plasma membrane was firmly attached to a substrate and the cytoplasmic face was exposed. The membrane patches contained densely packed particles whose diameter, after correcting for the geometry of the probing tip, was ∼10 nm. The particles were predominantly aligned unidirectionally with spacing of ∼36 nm. The density of the particle was ∼850 μm(−2), which could be an underestimate presumably due to the method of sample preparation. Antibody-labeled specimens showed particles more elevated than unlabeled preparation indicative of primary and secondary antibody complexes. The corrected diameters of these particles labeled with anti-actin were ∼12 nm while that with antiprestin were ∼8 nm. The alignment pattern in antiprestin-labeled specimens resembled that of the unlabeled preparation. Specimens labeled with actin antibodies did not show such alignment. We interpret that the particles observed in the unlabeled membranes correspond to the 10-nm particles reported by electron microscopy and that these particles contain prestin, a member of the SLC26 family, which is essential for electromotility. |
format | Text |
id | pubmed-2810367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-28103672011-02-01 Organization of membrane motor in outer hair cells: an atomic force microscopic study Sinha, Ghanshyam P. Sabri, Firouzeh Dimitriadis, Emilios K. Iwasa, Kuni H. Pflugers Arch Sensory Physiology Using atomic force microscopy, we imaged the cytosolic surface of the lateral plasma membrane of outer hair cells from guinea pigs’ inner ear. We used a “cell-free” preparation, in which a patch of plasma membrane was firmly attached to a substrate and the cytoplasmic face was exposed. The membrane patches contained densely packed particles whose diameter, after correcting for the geometry of the probing tip, was ∼10 nm. The particles were predominantly aligned unidirectionally with spacing of ∼36 nm. The density of the particle was ∼850 μm(−2), which could be an underestimate presumably due to the method of sample preparation. Antibody-labeled specimens showed particles more elevated than unlabeled preparation indicative of primary and secondary antibody complexes. The corrected diameters of these particles labeled with anti-actin were ∼12 nm while that with antiprestin were ∼8 nm. The alignment pattern in antiprestin-labeled specimens resembled that of the unlabeled preparation. Specimens labeled with actin antibodies did not show such alignment. We interpret that the particles observed in the unlabeled membranes correspond to the 10-nm particles reported by electron microscopy and that these particles contain prestin, a member of the SLC26 family, which is essential for electromotility. Springer-Verlag 2009-10-07 2010 /pmc/articles/PMC2810367/ /pubmed/19809831 http://dx.doi.org/10.1007/s00424-009-0742-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Sensory Physiology Sinha, Ghanshyam P. Sabri, Firouzeh Dimitriadis, Emilios K. Iwasa, Kuni H. Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title | Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title_full | Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title_fullStr | Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title_full_unstemmed | Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title_short | Organization of membrane motor in outer hair cells: an atomic force microscopic study |
title_sort | organization of membrane motor in outer hair cells: an atomic force microscopic study |
topic | Sensory Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810367/ https://www.ncbi.nlm.nih.gov/pubmed/19809831 http://dx.doi.org/10.1007/s00424-009-0742-3 |
work_keys_str_mv | AT sinhaghanshyamp organizationofmembranemotorinouterhaircellsanatomicforcemicroscopicstudy AT sabrifirouzeh organizationofmembranemotorinouterhaircellsanatomicforcemicroscopicstudy AT dimitriadisemiliosk organizationofmembranemotorinouterhaircellsanatomicforcemicroscopicstudy AT iwasakunih organizationofmembranemotorinouterhaircellsanatomicforcemicroscopicstudy |