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The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region

The C/D box scaRNA2 is predicted to guide specific 2′-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II an...

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Autores principales: Gérard, Marie-Aline, Myslinski, Evelyne, Chylak, Natassia, Baudrey, Stéphanie, Krol, Alain, Carbon, Philippe
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811027/
https://www.ncbi.nlm.nih.gov/pubmed/19906720
http://dx.doi.org/10.1093/nar/gkp988
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author Gérard, Marie-Aline
Myslinski, Evelyne
Chylak, Natassia
Baudrey, Stéphanie
Krol, Alain
Carbon, Philippe
author_facet Gérard, Marie-Aline
Myslinski, Evelyne
Chylak, Natassia
Baudrey, Stéphanie
Krol, Alain
Carbon, Philippe
author_sort Gérard, Marie-Aline
collection PubMed
description The C/D box scaRNA2 is predicted to guide specific 2′-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter elements responsible for its transcription are contained within a 161 bp region upstream of the transcription start site. In mammals, we have identified four cross species conserved promoter elements, a TATA motif, an hStaf/ZNF143 binding site and two novel elements that are required for full promoter activity. Binding of the human hStaf/ZNF143 transcription factor to its target sequence is required for promoter activity, suggesting that hStaf/ZNF143 is a fundamental regulator of the SCARNA2 gene. We also showed that RNA polymerase II continues transcription past the 3′-end of the mature RNA, irrespective of the identity of the Pol II promoter. The 3′-end processing and accumulation are governed by the sole information contained in the scaRNA2 encoding region, the maturation occurring via a particular pathway incompatible with that of mRNA or snRNA production.
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spelling pubmed-28110272010-01-26 The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region Gérard, Marie-Aline Myslinski, Evelyne Chylak, Natassia Baudrey, Stéphanie Krol, Alain Carbon, Philippe Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The C/D box scaRNA2 is predicted to guide specific 2′-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter elements responsible for its transcription are contained within a 161 bp region upstream of the transcription start site. In mammals, we have identified four cross species conserved promoter elements, a TATA motif, an hStaf/ZNF143 binding site and two novel elements that are required for full promoter activity. Binding of the human hStaf/ZNF143 transcription factor to its target sequence is required for promoter activity, suggesting that hStaf/ZNF143 is a fundamental regulator of the SCARNA2 gene. We also showed that RNA polymerase II continues transcription past the 3′-end of the mature RNA, irrespective of the identity of the Pol II promoter. The 3′-end processing and accumulation are governed by the sole information contained in the scaRNA2 encoding region, the maturation occurring via a particular pathway incompatible with that of mRNA or snRNA production. Oxford University Press 2010-01 2009-11-11 /pmc/articles/PMC2811027/ /pubmed/19906720 http://dx.doi.org/10.1093/nar/gkp988 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Gérard, Marie-Aline
Myslinski, Evelyne
Chylak, Natassia
Baudrey, Stéphanie
Krol, Alain
Carbon, Philippe
The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title_full The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title_fullStr The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title_full_unstemmed The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title_short The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region
title_sort scarna2 is produced by an independent transcription unit and its processing is directed by the encoding region
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811027/
https://www.ncbi.nlm.nih.gov/pubmed/19906720
http://dx.doi.org/10.1093/nar/gkp988
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