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Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutio...

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Autores principales: Kuwahara, Takashi, Kaneda, Shinya, Shimono, Kazuyuki, Inoue, Yoshifumi
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811814/
https://www.ncbi.nlm.nih.gov/pubmed/20107529
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author Kuwahara, Takashi
Kaneda, Shinya
Shimono, Kazuyuki
Inoue, Yoshifumi
author_facet Kuwahara, Takashi
Kaneda, Shinya
Shimono, Kazuyuki
Inoue, Yoshifumi
author_sort Kuwahara, Takashi
collection PubMed
description Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions without lipid. Methods: Experiment 1: A commercial TPN solution without lipid containing multivitamins (pH5.6) was used. A specific number of each test microorganism was added to each 10 mL of the TPN solution and incubated at room temperature. An aliquot of test solution was sampled and inoculated to SCD agar plates at 0, 24, and 48 hrs after the addition of the microorganisms. The number of microorganisms was counted as colony forming units. Experiment 2: The other 2 commercial TPN solutions without lipid (pH5.5) were supplemented with multivitamins. The pH values of the solutions were adjusted to about 6.0, 6.5, or 7.0 using 0.5 mol/L NaOH. The addition of microorganisms, incubation, and counting were performed in the same manner. Results: Experiment 1: S. aureus, S. marcescens, and B. cereus did not increase in the TPN solution without lipid containing multivitamins (pH5.6), but C. albicans increased rapidly. Experiment 2: The 3 bacterial species did not increase even at pH6.0, but increased at pH6.5 and increased rapidly at pH7.0 in both TPN solutions. C. albicans increased similarly at any pH. Conclusion: These results suggest that bacterial species cannot grow in TPN solutions without lipid due to the acidity (pH5.6 or lower), but Candida species can grow regardless of the acidity.
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spelling pubmed-28118142010-01-27 Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid Kuwahara, Takashi Kaneda, Shinya Shimono, Kazuyuki Inoue, Yoshifumi Int J Med Sci Research Paper Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions without lipid. Methods: Experiment 1: A commercial TPN solution without lipid containing multivitamins (pH5.6) was used. A specific number of each test microorganism was added to each 10 mL of the TPN solution and incubated at room temperature. An aliquot of test solution was sampled and inoculated to SCD agar plates at 0, 24, and 48 hrs after the addition of the microorganisms. The number of microorganisms was counted as colony forming units. Experiment 2: The other 2 commercial TPN solutions without lipid (pH5.5) were supplemented with multivitamins. The pH values of the solutions were adjusted to about 6.0, 6.5, or 7.0 using 0.5 mol/L NaOH. The addition of microorganisms, incubation, and counting were performed in the same manner. Results: Experiment 1: S. aureus, S. marcescens, and B. cereus did not increase in the TPN solution without lipid containing multivitamins (pH5.6), but C. albicans increased rapidly. Experiment 2: The 3 bacterial species did not increase even at pH6.0, but increased at pH6.5 and increased rapidly at pH7.0 in both TPN solutions. C. albicans increased similarly at any pH. Conclusion: These results suggest that bacterial species cannot grow in TPN solutions without lipid due to the acidity (pH5.6 or lower), but Candida species can grow regardless of the acidity. Ivyspring International Publisher 2010-01-22 /pmc/articles/PMC2811814/ /pubmed/20107529 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Kuwahara, Takashi
Kaneda, Shinya
Shimono, Kazuyuki
Inoue, Yoshifumi
Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title_full Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title_fullStr Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title_full_unstemmed Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title_short Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid
title_sort growth of microorganisms in total parenteral nutrition solutions without lipid
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811814/
https://www.ncbi.nlm.nih.gov/pubmed/20107529
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