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Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis

Cytoplasmic Cu/Zn superoxide dismutase (SOD1) is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production, allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wid...

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Autores principales: Yon, Jung-Min, Baek, In-Jeoung, Lee, Se-Ra, Kim, Mi-Ra, Lee, Beom Jun, Yun, Young Won, Nam, Sang-Yoon
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811834/
https://www.ncbi.nlm.nih.gov/pubmed/18716442
http://dx.doi.org/10.4142/jvs.2008.9.3.233
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author Yon, Jung-Min
Baek, In-Jeoung
Lee, Se-Ra
Kim, Mi-Ra
Lee, Beom Jun
Yun, Young Won
Nam, Sang-Yoon
author_facet Yon, Jung-Min
Baek, In-Jeoung
Lee, Se-Ra
Kim, Mi-Ra
Lee, Beom Jun
Yun, Young Won
Nam, Sang-Yoon
author_sort Yon, Jung-Min
collection PubMed
description Cytoplasmic Cu/Zn superoxide dismutase (SOD1) is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production, allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wide range of pathologies in mice, but little is known about normal SOD1 protein expression in developing embryos. In this study, the expression pattern of SOD1 was investigated in post-implantation mouse embryos and extraembryonic tissues, including placenta, using Western blotting and immunohistochemical analyses. SOD1 was detected in embryos and extraembryonic tissues from embryonic day (ED) 8.5 to 18.5. The signal in embryos was observed at the lowest level on ED 9.5-11.5, and the highest level on ED 17.5-18.5, while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED 13.5-18.5 revealed its ubiquitous distribution throughout developing organs. In particular, high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus, ganglia, sensory cells of the olfactory and vestibulocochlear epithelia, blood cells and vessels, hepatocytes and hematopoietic cells of the liver, lymph nodes, osteogenic tissues, and skin. Thus, SOD1 is highly expressed at late stages of embryonic development in a cell- and tissue-specific manner, and can function as an important antioxidant enzyme during organogenesis in mouse embryos.
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spelling pubmed-28118342010-01-28 Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis Yon, Jung-Min Baek, In-Jeoung Lee, Se-Ra Kim, Mi-Ra Lee, Beom Jun Yun, Young Won Nam, Sang-Yoon J Vet Sci Original Article Cytoplasmic Cu/Zn superoxide dismutase (SOD1) is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production, allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wide range of pathologies in mice, but little is known about normal SOD1 protein expression in developing embryos. In this study, the expression pattern of SOD1 was investigated in post-implantation mouse embryos and extraembryonic tissues, including placenta, using Western blotting and immunohistochemical analyses. SOD1 was detected in embryos and extraembryonic tissues from embryonic day (ED) 8.5 to 18.5. The signal in embryos was observed at the lowest level on ED 9.5-11.5, and the highest level on ED 17.5-18.5, while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED 13.5-18.5 revealed its ubiquitous distribution throughout developing organs. In particular, high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus, ganglia, sensory cells of the olfactory and vestibulocochlear epithelia, blood cells and vessels, hepatocytes and hematopoietic cells of the liver, lymph nodes, osteogenic tissues, and skin. Thus, SOD1 is highly expressed at late stages of embryonic development in a cell- and tissue-specific manner, and can function as an important antioxidant enzyme during organogenesis in mouse embryos. The Korean Society of Veterinary Science 2008-09 2008-09-30 /pmc/articles/PMC2811834/ /pubmed/18716442 http://dx.doi.org/10.4142/jvs.2008.9.3.233 Text en Copyright © 2008 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yon, Jung-Min
Baek, In-Jeoung
Lee, Se-Ra
Kim, Mi-Ra
Lee, Beom Jun
Yun, Young Won
Nam, Sang-Yoon
Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title_full Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title_fullStr Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title_full_unstemmed Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title_short Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis
title_sort immunohistochemical identification and quantitative analysis of cytoplasmic cu/zn superoxide dismutase in mouse organogenesis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811834/
https://www.ncbi.nlm.nih.gov/pubmed/18716442
http://dx.doi.org/10.4142/jvs.2008.9.3.233
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