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Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins

Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit v...

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Autores principales: Lee, Youn-Jeong, Sung, Haan-Woo, Choi, Jun-Gu, Lee, Eun-Kyoung, Yoon, Hachung, Kim, Jae-Hong, Song, Chang-Seon
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811843/
https://www.ncbi.nlm.nih.gov/pubmed/18716451
http://dx.doi.org/10.4142/jvs.2008.9.3.301
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author Lee, Youn-Jeong
Sung, Haan-Woo
Choi, Jun-Gu
Lee, Eun-Kyoung
Yoon, Hachung
Kim, Jae-Hong
Song, Chang-Seon
author_facet Lee, Youn-Jeong
Sung, Haan-Woo
Choi, Jun-Gu
Lee, Eun-Kyoung
Yoon, Hachung
Kim, Jae-Hong
Song, Chang-Seon
author_sort Lee, Youn-Jeong
collection PubMed
description Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program.
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spelling pubmed-28118432010-01-28 Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins Lee, Youn-Jeong Sung, Haan-Woo Choi, Jun-Gu Lee, Eun-Kyoung Yoon, Hachung Kim, Jae-Hong Song, Chang-Seon J Vet Sci Original Article Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program. The Korean Society of Veterinary Science 2008-09 2008-09-30 /pmc/articles/PMC2811843/ /pubmed/18716451 http://dx.doi.org/10.4142/jvs.2008.9.3.301 Text en Copyright © 2008 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Youn-Jeong
Sung, Haan-Woo
Choi, Jun-Gu
Lee, Eun-Kyoung
Yoon, Hachung
Kim, Jae-Hong
Song, Chang-Seon
Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title_full Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title_fullStr Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title_full_unstemmed Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title_short Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
title_sort protection of chickens from newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811843/
https://www.ncbi.nlm.nih.gov/pubmed/18716451
http://dx.doi.org/10.4142/jvs.2008.9.3.301
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