Analysis of Cooperativity by Isothermal Titration Calorimetry

Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affiniti...

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Detalles Bibliográficos
Autor principal: Brown, Alan
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812830/
https://www.ncbi.nlm.nih.gov/pubmed/20111687
http://dx.doi.org/10.3390/ijms10083457
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author Brown, Alan
author_facet Brown, Alan
author_sort Brown, Alan
collection PubMed
description Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.
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spelling pubmed-28128302010-01-28 Analysis of Cooperativity by Isothermal Titration Calorimetry Brown, Alan Int J Mol Sci Review Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems. Molecular Diversity Preservation International (MDPI) 2009-08-04 /pmc/articles/PMC2812830/ /pubmed/20111687 http://dx.doi.org/10.3390/ijms10083457 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Brown, Alan
Analysis of Cooperativity by Isothermal Titration Calorimetry
title Analysis of Cooperativity by Isothermal Titration Calorimetry
title_full Analysis of Cooperativity by Isothermal Titration Calorimetry
title_fullStr Analysis of Cooperativity by Isothermal Titration Calorimetry
title_full_unstemmed Analysis of Cooperativity by Isothermal Titration Calorimetry
title_short Analysis of Cooperativity by Isothermal Titration Calorimetry
title_sort analysis of cooperativity by isothermal titration calorimetry
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812830/
https://www.ncbi.nlm.nih.gov/pubmed/20111687
http://dx.doi.org/10.3390/ijms10083457
work_keys_str_mv AT brownalan analysisofcooperativitybyisothermaltitrationcalorimetry

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