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Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography

The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles...

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Detalles Bibliográficos
Autores principales: Fernández-Busnadiego, Rubén, Zuber, Benoît, Maurer, Ulrike Elisabeth, Cyrklaff, Marek, Baumeister, Wolfgang, Lučić, Vladan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812849/
https://www.ncbi.nlm.nih.gov/pubmed/20065095
http://dx.doi.org/10.1083/jcb.200908082
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author Fernández-Busnadiego, Rubén
Zuber, Benoît
Maurer, Ulrike Elisabeth
Cyrklaff, Marek
Baumeister, Wolfgang
Lučić, Vladan
author_facet Fernández-Busnadiego, Rubén
Zuber, Benoît
Maurer, Ulrike Elisabeth
Cyrklaff, Marek
Baumeister, Wolfgang
Lučić, Vladan
author_sort Fernández-Busnadiego, Rubén
collection PubMed
description The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release.
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spelling pubmed-28128492010-07-11 Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography Fernández-Busnadiego, Rubén Zuber, Benoît Maurer, Ulrike Elisabeth Cyrklaff, Marek Baumeister, Wolfgang Lučić, Vladan J Cell Biol Research Articles The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release. The Rockefeller University Press 2010-01-11 /pmc/articles/PMC2812849/ /pubmed/20065095 http://dx.doi.org/10.1083/jcb.200908082 Text en © 2010 Fernández-Busnadiego et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Fernández-Busnadiego, Rubén
Zuber, Benoît
Maurer, Ulrike Elisabeth
Cyrklaff, Marek
Baumeister, Wolfgang
Lučić, Vladan
Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title_full Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title_fullStr Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title_full_unstemmed Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title_short Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
title_sort quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812849/
https://www.ncbi.nlm.nih.gov/pubmed/20065095
http://dx.doi.org/10.1083/jcb.200908082
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