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Non-imprinted allele-specific DNA methylation on human autosomes

BACKGROUND: Differential DNA methylation between alleles is well established in imprinted genes and the X chromosomes in females but has rarely been reported at non-imprinted loci on autosomes. RESULTS: We studied DNA methylation of cytosine-guanine dinucleotide (CpG) islands on chromosome 21 in leu...

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Autores principales: Zhang, Yingying, Rohde, Christian, Reinhardt, Richard, Voelcker-Rehage, Claudia, Jeltsch, Albert
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812945/
https://www.ncbi.nlm.nih.gov/pubmed/19958531
http://dx.doi.org/10.1186/gb-2009-10-12-r138
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author Zhang, Yingying
Rohde, Christian
Reinhardt, Richard
Voelcker-Rehage, Claudia
Jeltsch, Albert
author_facet Zhang, Yingying
Rohde, Christian
Reinhardt, Richard
Voelcker-Rehage, Claudia
Jeltsch, Albert
author_sort Zhang, Yingying
collection PubMed
description BACKGROUND: Differential DNA methylation between alleles is well established in imprinted genes and the X chromosomes in females but has rarely been reported at non-imprinted loci on autosomes. RESULTS: We studied DNA methylation of cytosine-guanine dinucleotide (CpG) islands on chromosome 21 in leukocytes from several healthy individuals and observed novel cases of pronounced differential methylation of alleles. Allele-specific methylation affected complete CpG islands with methylation differences between alleles of up to 85%. The methylation differences between alleles were strongly correlated with the genotypes, excluding a connection to imprinting. We show that allele-specific methylation can lead to allelic repression of the methylated gene copy. Based on our results, allele-specific methylation is likely to affect about 10% of all human genes and to contribute to allele-specific expression and monoallelic gene silencing. Therefore, allele-specific methylation represents an epigenetic pathway of how genetic polymorphisms may lead to phenotypic variability. In most cases, we observed that some, but not all, heterozygous individuals showed allele-specific methylation, suggesting that allele-specific methylation is the outcome of an epigenetic drift, the direction of which is determined by the genetic differences between the alleles. We could show that the tendency to acquire hypermethylation in one allele was inherited. CONCLUSIONS: We observed that larger differences in methylation levels between individuals were often coupled to allele-specific methylation and genetic polymorphisms, suggesting that the inter-individual variability of DNA methylation is strongly influenced by genetic differences. Therefore, genetic differences must be taken into account in future comparative DNA methylation studies.
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spelling pubmed-28129452010-01-29 Non-imprinted allele-specific DNA methylation on human autosomes Zhang, Yingying Rohde, Christian Reinhardt, Richard Voelcker-Rehage, Claudia Jeltsch, Albert Genome Biol Research BACKGROUND: Differential DNA methylation between alleles is well established in imprinted genes and the X chromosomes in females but has rarely been reported at non-imprinted loci on autosomes. RESULTS: We studied DNA methylation of cytosine-guanine dinucleotide (CpG) islands on chromosome 21 in leukocytes from several healthy individuals and observed novel cases of pronounced differential methylation of alleles. Allele-specific methylation affected complete CpG islands with methylation differences between alleles of up to 85%. The methylation differences between alleles were strongly correlated with the genotypes, excluding a connection to imprinting. We show that allele-specific methylation can lead to allelic repression of the methylated gene copy. Based on our results, allele-specific methylation is likely to affect about 10% of all human genes and to contribute to allele-specific expression and monoallelic gene silencing. Therefore, allele-specific methylation represents an epigenetic pathway of how genetic polymorphisms may lead to phenotypic variability. In most cases, we observed that some, but not all, heterozygous individuals showed allele-specific methylation, suggesting that allele-specific methylation is the outcome of an epigenetic drift, the direction of which is determined by the genetic differences between the alleles. We could show that the tendency to acquire hypermethylation in one allele was inherited. CONCLUSIONS: We observed that larger differences in methylation levels between individuals were often coupled to allele-specific methylation and genetic polymorphisms, suggesting that the inter-individual variability of DNA methylation is strongly influenced by genetic differences. Therefore, genetic differences must be taken into account in future comparative DNA methylation studies. BioMed Central 2009 2009-12-03 /pmc/articles/PMC2812945/ /pubmed/19958531 http://dx.doi.org/10.1186/gb-2009-10-12-r138 Text en Copyright ©2009 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhang, Yingying
Rohde, Christian
Reinhardt, Richard
Voelcker-Rehage, Claudia
Jeltsch, Albert
Non-imprinted allele-specific DNA methylation on human autosomes
title Non-imprinted allele-specific DNA methylation on human autosomes
title_full Non-imprinted allele-specific DNA methylation on human autosomes
title_fullStr Non-imprinted allele-specific DNA methylation on human autosomes
title_full_unstemmed Non-imprinted allele-specific DNA methylation on human autosomes
title_short Non-imprinted allele-specific DNA methylation on human autosomes
title_sort non-imprinted allele-specific dna methylation on human autosomes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812945/
https://www.ncbi.nlm.nih.gov/pubmed/19958531
http://dx.doi.org/10.1186/gb-2009-10-12-r138
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