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Performance of serum-supplemented and serum-free media in IFNγ Elispot Assays for human T cells
The choice of serum for supplementation of media for T cell assays and in particular, Elispot has been a major challenge for assay performance, standardization, optimization, and reproducibility. The Assay Working Group of the Cancer Vaccine Consortium (CVC-CRI) has recently identified the choice of...
Autores principales: | Janetzki, Sylvia, Price, L., Britten, C. M., van der Burg, S. H., Caterini, J., Currier, J. R., Ferrari, G., Gouttefangeas, C., Hayes, P., Kaempgen, E., Lennerz, V., Nihlmark, K., Souza, V., Hoos, A. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2813531/ https://www.ncbi.nlm.nih.gov/pubmed/19894047 http://dx.doi.org/10.1007/s00262-009-0788-2 |
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