Isolation of Helicobacter pylori from dental plaque: A microbiological study

AIM: The aim of our study was to isolate H. pylori from dental plaque in gastric and duodenal ulcer patients and compare it with dental plaque of healthy subjects. MATERIALS AND METHODS: Fifty patients in the age range of 25-50 years who were endoscopically proven cases of duodenal and gastric ulcer were chosen. H. pylorus was isolated from the dental plaque of these patients using culture method and rapid urease test (RUT). It was compared with the dental plaque from control group (25 students). The specificity and sensitivity of RUT was compared with culture method. The oral hygiene index (OHI) score and plaque index were assessed. RESULTS: Ten percent positivity was observed in the study group by culture. Though RUT showed 70% positive isolation it is neither a specific test nor a conclusive test as compared to culture. The result correlates with oral hygiene in study population. CONCLUSION: Further, more studies are needed to compare RUT and culture, with serology and polymerase chain reactions. The ability to detect H. pylori from dental plaque using these methods offer the potential for the noninvasive test for infection and would aid in support of oral transmission of H. pylori.