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Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells

The obligate intracellular microsporidia have developed a unique invasion mechanism to infect their host cells. Spores explosively evert a tube-like structure and extrude the infectious spore content through this organelle into the host cell. Spores from species of the genus Encephalitozoon were als...

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Autores principales: Orlik, Johanna, Böttcher, Karin, Groß, Uwe, Bohne, Wolfgang
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815800/
https://www.ncbi.nlm.nih.gov/pubmed/20107836
http://dx.doi.org/10.1007/s00436-010-1736-y
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author Orlik, Johanna
Böttcher, Karin
Groß, Uwe
Bohne, Wolfgang
author_facet Orlik, Johanna
Böttcher, Karin
Groß, Uwe
Bohne, Wolfgang
author_sort Orlik, Johanna
collection PubMed
description The obligate intracellular microsporidia have developed a unique invasion mechanism to infect their host cells. Spores explosively evert a tube-like structure and extrude the infectious spore content through this organelle into the host cell. Spores from species of the genus Encephalitozoon were also shown to be efficiently internalized by phagocytosis, which led to the hypothesis that spore germination from inside a phagosome might contribute to the infection process. Here, we challenge this hypothesis by quantifying Encephalitozoon cuniculi infection rates of J774 cells that were incubated with the phagocytosis inhibitor cytochalasin D. We demonstrate that the invasion rate in cytochalasin D-treated cells is identical to untreated controls, although phagocytic uptake of E. cuniculi spores was less than 10% of control samples. This study suggests that germination of phagocytosed spores is not a significant infection mode for E. cuniculi.
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spelling pubmed-28158002010-02-13 Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells Orlik, Johanna Böttcher, Karin Groß, Uwe Bohne, Wolfgang Parasitol Res Short Communication The obligate intracellular microsporidia have developed a unique invasion mechanism to infect their host cells. Spores explosively evert a tube-like structure and extrude the infectious spore content through this organelle into the host cell. Spores from species of the genus Encephalitozoon were also shown to be efficiently internalized by phagocytosis, which led to the hypothesis that spore germination from inside a phagosome might contribute to the infection process. Here, we challenge this hypothesis by quantifying Encephalitozoon cuniculi infection rates of J774 cells that were incubated with the phagocytosis inhibitor cytochalasin D. We demonstrate that the invasion rate in cytochalasin D-treated cells is identical to untreated controls, although phagocytic uptake of E. cuniculi spores was less than 10% of control samples. This study suggests that germination of phagocytosed spores is not a significant infection mode for E. cuniculi. Springer-Verlag 2010-01-28 2010 /pmc/articles/PMC2815800/ /pubmed/20107836 http://dx.doi.org/10.1007/s00436-010-1736-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Short Communication
Orlik, Johanna
Böttcher, Karin
Groß, Uwe
Bohne, Wolfgang
Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title_full Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title_fullStr Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title_full_unstemmed Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title_short Germination of phagocytosed E. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in J774 cells
title_sort germination of phagocytosed e. cuniculi spores does not significantly contribute to parasitophorous vacuole formation in j774 cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815800/
https://www.ncbi.nlm.nih.gov/pubmed/20107836
http://dx.doi.org/10.1007/s00436-010-1736-y
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