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Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death

BACKGROUND: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell prol...

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Autores principales: Song, Hyunkeun, Hur, Indo, Park, Hyun-jin, Nam, Joohyung, Park, Ga Bin, Kong, Kyoung Hye, Hwang, Young Mi, Kim, Yeong Seok, Cho, Dae Ho, Lee, Wang Jae, Hur, Dae Young
Formato: Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816956/
https://www.ncbi.nlm.nih.gov/pubmed/20157610
http://dx.doi.org/10.4110/in.2009.9.6.236
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author Song, Hyunkeun
Hur, Indo
Park, Hyun-jin
Nam, Joohyung
Park, Ga Bin
Kong, Kyoung Hye
Hwang, Young Mi
Kim, Yeong Seok
Cho, Dae Ho
Lee, Wang Jae
Hur, Dae Young
author_facet Song, Hyunkeun
Hur, Indo
Park, Hyun-jin
Nam, Joohyung
Park, Ga Bin
Kong, Kyoung Hye
Hwang, Young Mi
Kim, Yeong Seok
Cho, Dae Ho
Lee, Wang Jae
Hur, Dae Young
author_sort Song, Hyunkeun
collection PubMed
description BACKGROUND: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. METHODS: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model. RESULTS: Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model. CONCLUSION: These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells.
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spelling pubmed-28169562010-02-12 Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death Song, Hyunkeun Hur, Indo Park, Hyun-jin Nam, Joohyung Park, Ga Bin Kong, Kyoung Hye Hwang, Young Mi Kim, Yeong Seok Cho, Dae Ho Lee, Wang Jae Hur, Dae Young Immune Netw Original Article BACKGROUND: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. METHODS: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model. RESULTS: Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model. CONCLUSION: These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells. The Korean Association of Immunologists 2009-12 2009-12-31 /pmc/articles/PMC2816956/ /pubmed/20157610 http://dx.doi.org/10.4110/in.2009.9.6.236 Text en Copyright © 2009 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Song, Hyunkeun
Hur, Indo
Park, Hyun-jin
Nam, Joohyung
Park, Ga Bin
Kong, Kyoung Hye
Hwang, Young Mi
Kim, Yeong Seok
Cho, Dae Ho
Lee, Wang Jae
Hur, Dae Young
Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title_full Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title_fullStr Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title_full_unstemmed Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title_short Selenium Inhibits Metastasis of Murine Melanoma Cells through the Induction of Cell Cycle Arrest and Cell Death
title_sort selenium inhibits metastasis of murine melanoma cells through the induction of cell cycle arrest and cell death
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816956/
https://www.ncbi.nlm.nih.gov/pubmed/20157610
http://dx.doi.org/10.4110/in.2009.9.6.236
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