Cargando…
Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling
BACKGROUND: Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo. METHODS: The expression of cytokines and swiprosin-1 were determined by by...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Korean Association of Immunologists
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816961/ https://www.ncbi.nlm.nih.gov/pubmed/20157615 http://dx.doi.org/10.4110/in.2009.9.6.274 |
_version_ | 1782177161428336640 |
---|---|
author | Ramesh, T.P. Kim, Young-Dae Kwon, Min-Sung Jun, Chang-Duk Kim, Sang-Wook |
author_facet | Ramesh, T.P. Kim, Young-Dae Kwon, Min-Sung Jun, Chang-Duk Kim, Sang-Wook |
author_sort | Ramesh, T.P. |
collection | PubMed |
description | BACKGROUND: Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo. METHODS: The expression of cytokines and swiprosin-1 were determined by by real time PCR and conventional PCR. Pharmacological inhibitors were treated to investigate potential mechanism of swiprosin-1 in mast cell activation. Actin content was evaluated by confocal microscopy and flow cytometry. RESULTS: The swiprosin-1 augmented PMA/A23187-induced expression of cytokines and release of histamine. However, knock-down of swiprosin-1 showed only a modest effect on PMA/A23187-induced cytokine expression, suggesting that swiprosin-1 has gain-of-function characteristics. Swiprosin-1 was found in microvilli-like membrane protrusions and highly co-localized with F-actin. Importantly, either disruption of actin by cytochalasin B or inhibition of PI3 kinase, an enzyme involved in actin remodeling, by wortmannin blocked cytokine expression only in swiprosin-1-overexpressing cells. CONCLUSION: These results suggest that swiprosin-1 modulates mast cell activation potentially through actin regulation. |
format | Text |
id | pubmed-2816961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Korean Association of Immunologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-28169612010-02-12 Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling Ramesh, T.P. Kim, Young-Dae Kwon, Min-Sung Jun, Chang-Duk Kim, Sang-Wook Immune Netw Original Article BACKGROUND: Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo. METHODS: The expression of cytokines and swiprosin-1 were determined by by real time PCR and conventional PCR. Pharmacological inhibitors were treated to investigate potential mechanism of swiprosin-1 in mast cell activation. Actin content was evaluated by confocal microscopy and flow cytometry. RESULTS: The swiprosin-1 augmented PMA/A23187-induced expression of cytokines and release of histamine. However, knock-down of swiprosin-1 showed only a modest effect on PMA/A23187-induced cytokine expression, suggesting that swiprosin-1 has gain-of-function characteristics. Swiprosin-1 was found in microvilli-like membrane protrusions and highly co-localized with F-actin. Importantly, either disruption of actin by cytochalasin B or inhibition of PI3 kinase, an enzyme involved in actin remodeling, by wortmannin blocked cytokine expression only in swiprosin-1-overexpressing cells. CONCLUSION: These results suggest that swiprosin-1 modulates mast cell activation potentially through actin regulation. The Korean Association of Immunologists 2009-12 2009-12-31 /pmc/articles/PMC2816961/ /pubmed/20157615 http://dx.doi.org/10.4110/in.2009.9.6.274 Text en Copyright © 2009 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ramesh, T.P. Kim, Young-Dae Kwon, Min-Sung Jun, Chang-Duk Kim, Sang-Wook Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title | Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title_full | Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title_fullStr | Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title_full_unstemmed | Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title_short | Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling |
title_sort | swiprosin-1 regulates cytokine expression of human mast cell line hmc-1 through actin remodeling |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816961/ https://www.ncbi.nlm.nih.gov/pubmed/20157615 http://dx.doi.org/10.4110/in.2009.9.6.274 |
work_keys_str_mv | AT rameshtp swiprosin1regulatescytokineexpressionofhumanmastcelllinehmc1throughactinremodeling AT kimyoungdae swiprosin1regulatescytokineexpressionofhumanmastcelllinehmc1throughactinremodeling AT kwonminsung swiprosin1regulatescytokineexpressionofhumanmastcelllinehmc1throughactinremodeling AT junchangduk swiprosin1regulatescytokineexpressionofhumanmastcelllinehmc1throughactinremodeling AT kimsangwook swiprosin1regulatescytokineexpressionofhumanmastcelllinehmc1throughactinremodeling |