Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas

PURPOSE: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-β isoforms in bullous keratopathy corneas. METHODS: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included...

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Detalles Bibliográficos
Autores principales: Strzalka-Mrozik, Barbara, Stanik-Walentek, Agnieszka, Kapral, Malgorzata, Kowalczyk, Malgorzata, Adamska, Jolanta, Gola, Joanna, Mazurek, Urszula
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817012/
https://www.ncbi.nlm.nih.gov/pubmed/20142847
Descripción
Sumario:PURPOSE: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-β isoforms in bullous keratopathy corneas. METHODS: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included 45 corneal donors (21 females and 24 males). Quantification of TGF-β1, TGF-β2, and TGF-β3 mRNAs was performed by real-time quantitative reverse transcription PCR (QRT-PCR). RESULTS: TGF-β1, TGF-β2, and TGF-β3 mRNAs were detected in both normal and pseudophakic bullous keratopathy (PBK) corneas. We found significantly lower transcriptional activity of TGF-β3 mRNA in bullous keratopathy corneas compared to normal tissues. TGF-β1 and TGF-β2 expressions were at the same level in both PBK and healthy corneas. CONCLUSIONS: Downregulation of TGF-β3 gene expression may play a significant role in molecular changes observed in bullous keratopathy.

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