Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas

PURPOSE: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-β isoforms in bullous keratopathy corneas. METHODS: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included...

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Autores principales: Strzalka-Mrozik, Barbara, Stanik-Walentek, Agnieszka, Kapral, Malgorzata, Kowalczyk, Malgorzata, Adamska, Jolanta, Gola, Joanna, Mazurek, Urszula
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817012/
https://www.ncbi.nlm.nih.gov/pubmed/20142847
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author Strzalka-Mrozik, Barbara
Stanik-Walentek, Agnieszka
Kapral, Malgorzata
Kowalczyk, Malgorzata
Adamska, Jolanta
Gola, Joanna
Mazurek, Urszula
author_facet Strzalka-Mrozik, Barbara
Stanik-Walentek, Agnieszka
Kapral, Malgorzata
Kowalczyk, Malgorzata
Adamska, Jolanta
Gola, Joanna
Mazurek, Urszula
author_sort Strzalka-Mrozik, Barbara
collection PubMed
description PURPOSE: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-β isoforms in bullous keratopathy corneas. METHODS: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included 45 corneal donors (21 females and 24 males). Quantification of TGF-β1, TGF-β2, and TGF-β3 mRNAs was performed by real-time quantitative reverse transcription PCR (QRT-PCR). RESULTS: TGF-β1, TGF-β2, and TGF-β3 mRNAs were detected in both normal and pseudophakic bullous keratopathy (PBK) corneas. We found significantly lower transcriptional activity of TGF-β3 mRNA in bullous keratopathy corneas compared to normal tissues. TGF-β1 and TGF-β2 expressions were at the same level in both PBK and healthy corneas. CONCLUSIONS: Downregulation of TGF-β3 gene expression may play a significant role in molecular changes observed in bullous keratopathy.
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spelling pubmed-28170122010-02-08 Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas Strzalka-Mrozik, Barbara Stanik-Walentek, Agnieszka Kapral, Malgorzata Kowalczyk, Malgorzata Adamska, Jolanta Gola, Joanna Mazurek, Urszula Mol Vis Research Article PURPOSE: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-β isoforms in bullous keratopathy corneas. METHODS: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included 45 corneal donors (21 females and 24 males). Quantification of TGF-β1, TGF-β2, and TGF-β3 mRNAs was performed by real-time quantitative reverse transcription PCR (QRT-PCR). RESULTS: TGF-β1, TGF-β2, and TGF-β3 mRNAs were detected in both normal and pseudophakic bullous keratopathy (PBK) corneas. We found significantly lower transcriptional activity of TGF-β3 mRNA in bullous keratopathy corneas compared to normal tissues. TGF-β1 and TGF-β2 expressions were at the same level in both PBK and healthy corneas. CONCLUSIONS: Downregulation of TGF-β3 gene expression may play a significant role in molecular changes observed in bullous keratopathy. Molecular Vision 2010-02-05 /pmc/articles/PMC2817012/ /pubmed/20142847 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Strzalka-Mrozik, Barbara
Stanik-Walentek, Agnieszka
Kapral, Malgorzata
Kowalczyk, Malgorzata
Adamska, Jolanta
Gola, Joanna
Mazurek, Urszula
Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title_full Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title_fullStr Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title_full_unstemmed Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title_short Differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
title_sort differential expression of transforming growth factor-β isoforms in bullous keratopathy corneas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817012/
https://www.ncbi.nlm.nih.gov/pubmed/20142847
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