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Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body
PURPOSE: To investigate the relationships among antioxidant activities, oxidative stress, and vascular endothelial growth factor (VEGF) in the vitreous body and serum from proliferative diabetic retinopathy (PDR) patients. METHODS: In 21 patients with PDR and 21 controls with macular hole (MH), the...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817014/ https://www.ncbi.nlm.nih.gov/pubmed/20142849 |
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author | Izuta, Hiroshi Matsunaga, Nozomu Shimazawa, Masamitsu Sugiyama, Tetsuya Ikeda, Tsunehiko Hara, Hideaki |
author_facet | Izuta, Hiroshi Matsunaga, Nozomu Shimazawa, Masamitsu Sugiyama, Tetsuya Ikeda, Tsunehiko Hara, Hideaki |
author_sort | Izuta, Hiroshi |
collection | PubMed |
description | PURPOSE: To investigate the relationships among antioxidant activities, oxidative stress, and vascular endothelial growth factor (VEGF) in the vitreous body and serum from proliferative diabetic retinopathy (PDR) patients. METHODS: In 21 patients with PDR and 21 controls with macular hole (MH), the VEGF and lipid peroxide (Nε-hexanoyl-lysine [HEL]) levels in the vitreous and serum were measured by enzyme-linked immunosorbent assay, while antioxidant capacity (potential antioxidant [PAO]) was measured by chemical reduction of Cu(2+). RESULTS: Both the PAO and HEL levels in the vitreous and serum were significantly higher in PDR patients than in those with MH (both p<0.01). The VEGF concentrations in the vitreous were higher in PDR patients than in those with MH (p<0.01); however, the VEGF concentrations in the serum were not different between the two groups (p=0.95). Positive correlations were found between the PAO and VEGF concentrations and between the HEL and VEGF concentrations in the vitreous of both the PDR and the MH patients. CONCLUSIONS: Our study revealed that the PAO, HEL, and VEGF concentrations in the vitreous were increased in PDR versus MH patients and that there were positive correlations among these factors. This is consistent with VEGF and lipid peroxide levels in the vitreous playing some role in the pathogenesis of PDR. |
format | Text |
id | pubmed-2817014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-28170142010-02-08 Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body Izuta, Hiroshi Matsunaga, Nozomu Shimazawa, Masamitsu Sugiyama, Tetsuya Ikeda, Tsunehiko Hara, Hideaki Mol Vis Research Article PURPOSE: To investigate the relationships among antioxidant activities, oxidative stress, and vascular endothelial growth factor (VEGF) in the vitreous body and serum from proliferative diabetic retinopathy (PDR) patients. METHODS: In 21 patients with PDR and 21 controls with macular hole (MH), the VEGF and lipid peroxide (Nε-hexanoyl-lysine [HEL]) levels in the vitreous and serum were measured by enzyme-linked immunosorbent assay, while antioxidant capacity (potential antioxidant [PAO]) was measured by chemical reduction of Cu(2+). RESULTS: Both the PAO and HEL levels in the vitreous and serum were significantly higher in PDR patients than in those with MH (both p<0.01). The VEGF concentrations in the vitreous were higher in PDR patients than in those with MH (p<0.01); however, the VEGF concentrations in the serum were not different between the two groups (p=0.95). Positive correlations were found between the PAO and VEGF concentrations and between the HEL and VEGF concentrations in the vitreous of both the PDR and the MH patients. CONCLUSIONS: Our study revealed that the PAO, HEL, and VEGF concentrations in the vitreous were increased in PDR versus MH patients and that there were positive correlations among these factors. This is consistent with VEGF and lipid peroxide levels in the vitreous playing some role in the pathogenesis of PDR. Molecular Vision 2010-01-29 /pmc/articles/PMC2817014/ /pubmed/20142849 Text en Copyright © 2010 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Izuta, Hiroshi Matsunaga, Nozomu Shimazawa, Masamitsu Sugiyama, Tetsuya Ikeda, Tsunehiko Hara, Hideaki Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title | Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title_full | Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title_fullStr | Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title_full_unstemmed | Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title_short | Proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and VEGF in the vitreous body |
title_sort | proliferative diabetic retinopathy and relations among antioxidant activity, oxidative stress, and vegf in the vitreous body |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817014/ https://www.ncbi.nlm.nih.gov/pubmed/20142849 |
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