A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5′ RACE and a molecular beacon probe

Specific detection of mRNA cleavage by 5′RACE is the only method to confirm the knockdown of mRNA by RNA interference, but is rarely reported for in vivo studies. We have combined 5′-RNA-linker-mediated RACE (5′-RLM-RACE) with real-time PCR using a molecular beacon to develop a rapid and specific me...

Descripción completa

Detalles Bibliográficos
Autores principales: Lasham, Annette, Herbert, Mike, Coppieters ‘t Wallant, Natacha, Patel, Rachna, Feng, Sheryl, Eszes, Marika, Cao, Helen, Reid, Glen
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817477/
https://www.ncbi.nlm.nih.gov/pubmed/19942683
http://dx.doi.org/10.1093/nar/gkp1076

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817477/
https://www.ncbi.nlm.nih.gov/pubmed/19942683
http://dx.doi.org/10.1093/nar/gkp1076

Ejemplares similares