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Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)

Over ninety percent of the value of cotton comes from its fiber; however, the genetic mechanisms governing fiber development are poorly understood. Due to their biochemical and morphological diversity in fiber cells cotton fiber mutants have been useful in examining fiber development; therefore, usi...

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Detalles Bibliográficos
Autores principales: Bolton, James J., Soliman, Khairy M., Wilkins, Thea A., Jenkins, Johnie N.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817544/
https://www.ncbi.nlm.nih.gov/pubmed/20148073
http://dx.doi.org/10.1155/2009/659301
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author Bolton, James J.
Soliman, Khairy M.
Wilkins, Thea A.
Jenkins, Johnie N.
author_facet Bolton, James J.
Soliman, Khairy M.
Wilkins, Thea A.
Jenkins, Johnie N.
author_sort Bolton, James J.
collection PubMed
description Over ninety percent of the value of cotton comes from its fiber; however, the genetic mechanisms governing fiber development are poorly understood. Due to their biochemical and morphological diversity in fiber cells cotton fiber mutants have been useful in examining fiber development; therefore, using the Ligon Lintless (L i-1) mutant, a monogenic dominant cotton mutant with very short fibers, we employed the high throughput approaches of microarray technology and real time PCR to gain insights into what genes were critical during the secondary cell wall synthesis stage. Comparative transcriptome analysis of the normal TM-1 genotype and the near isogenic L i-1 revealed that over 100 transcripts were differentially expressed at least 2-fold during secondary wall biogenesis, although the genetic profile of the expansion phase showed no significant differences in the isolines. Of particular note, we identified three candidate gene families-expansin, sucrose synthase, and tubulin—whose expression in L i-1 deviates from normal expression patterns of its parent, TM-1. These genes may contribute to retarded growth of fibers in L i-1 since they are fiber-expressed structural and metabolic genes. This work provides more details into the mechanisms of fiber development, and suggests the L i gene is active during the later stages of fiber development.
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spelling pubmed-28175442010-02-10 Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1) Bolton, James J. Soliman, Khairy M. Wilkins, Thea A. Jenkins, Johnie N. Comp Funct Genomics Research Article Over ninety percent of the value of cotton comes from its fiber; however, the genetic mechanisms governing fiber development are poorly understood. Due to their biochemical and morphological diversity in fiber cells cotton fiber mutants have been useful in examining fiber development; therefore, using the Ligon Lintless (L i-1) mutant, a monogenic dominant cotton mutant with very short fibers, we employed the high throughput approaches of microarray technology and real time PCR to gain insights into what genes were critical during the secondary cell wall synthesis stage. Comparative transcriptome analysis of the normal TM-1 genotype and the near isogenic L i-1 revealed that over 100 transcripts were differentially expressed at least 2-fold during secondary wall biogenesis, although the genetic profile of the expansion phase showed no significant differences in the isolines. Of particular note, we identified three candidate gene families-expansin, sucrose synthase, and tubulin—whose expression in L i-1 deviates from normal expression patterns of its parent, TM-1. These genes may contribute to retarded growth of fibers in L i-1 since they are fiber-expressed structural and metabolic genes. This work provides more details into the mechanisms of fiber development, and suggests the L i gene is active during the later stages of fiber development. Hindawi Publishing Corporation 2009 2010-01-28 /pmc/articles/PMC2817544/ /pubmed/20148073 http://dx.doi.org/10.1155/2009/659301 Text en Copyright © 2009 James J. Bolton et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bolton, James J.
Soliman, Khairy M.
Wilkins, Thea A.
Jenkins, Johnie N.
Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title_full Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title_fullStr Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title_full_unstemmed Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title_short Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (L i-1)
title_sort aberrant expression of critical genes during secondary cell wall biogenesis in a cotton mutant, ligon lintless-1 (l i-1)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817544/
https://www.ncbi.nlm.nih.gov/pubmed/20148073
http://dx.doi.org/10.1155/2009/659301
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