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Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies

Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitati...

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Autores principales: Park, Hyejung, Haynes, Christopher A., Nairn, Alison V., Kulik, Michael, Dalton, Stephen, Moremen, Kelley, Merrill, Alfred H.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817578/
https://www.ncbi.nlm.nih.gov/pubmed/19786568
http://dx.doi.org/10.1194/jlr.M000984
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author Park, Hyejung
Haynes, Christopher A.
Nairn, Alison V.
Kulik, Michael
Dalton, Stephen
Moremen, Kelley
Merrill, Alfred H.
author_facet Park, Hyejung
Haynes, Christopher A.
Nairn, Alison V.
Kulik, Michael
Dalton, Stephen
Moremen, Kelley
Merrill, Alfred H.
author_sort Park, Hyejung
collection PubMed
description Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18- and C≥24-carbons dihydroceramides (DH)Cer, respectively; EBs have higher CerS2 (for C24:0- and C24:1-); and EBs have lower CerS5 + CerS6 (for C16-). In agreement with these findings, EBs have (DH)Cer with higher proportions of C18-, C24- and C26- and less C16-fatty acids, and longer (DH)Cer are also seen in monohexosylCers and sphingomyelins. EBs had higher mRNAs for fatty acyl-CoA elongases that produce C18-, C24-, and C26-fatty acyl-CoAs (Elovl3 and Elovl6), and higher amounts of these cosubstrates for CerS. Thus, these studies have found generally good agreement between genomic and metabolomic data in defining that conversion of mESCs to EBs is accompanied by a large number of changes in gene expression and subspecies distributions for both sphingolipids and fatty acyl-CoAs.
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spelling pubmed-28175782010-03-01 Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies Park, Hyejung Haynes, Christopher A. Nairn, Alison V. Kulik, Michael Dalton, Stephen Moremen, Kelley Merrill, Alfred H. J Lipid Res Research Article Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the Cer amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring the mRNA levels by quantitative RT-PCR and the amounts of the respective metabolites by LC-ESI/MS/MS, notable differences between R1 mESCs and EBs were: EBs have higher mRNAs for CerS1 and CerS3, which synthesize C18- and C≥24-carbons dihydroceramides (DH)Cer, respectively; EBs have higher CerS2 (for C24:0- and C24:1-); and EBs have lower CerS5 + CerS6 (for C16-). In agreement with these findings, EBs have (DH)Cer with higher proportions of C18-, C24- and C26- and less C16-fatty acids, and longer (DH)Cer are also seen in monohexosylCers and sphingomyelins. EBs had higher mRNAs for fatty acyl-CoA elongases that produce C18-, C24-, and C26-fatty acyl-CoAs (Elovl3 and Elovl6), and higher amounts of these cosubstrates for CerS. Thus, these studies have found generally good agreement between genomic and metabolomic data in defining that conversion of mESCs to EBs is accompanied by a large number of changes in gene expression and subspecies distributions for both sphingolipids and fatty acyl-CoAs. The American Society for Biochemistry and Molecular Biology 2010-03 /pmc/articles/PMC2817578/ /pubmed/19786568 http://dx.doi.org/10.1194/jlr.M000984 Text en Copyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articles
spellingShingle Research Article
Park, Hyejung
Haynes, Christopher A.
Nairn, Alison V.
Kulik, Michael
Dalton, Stephen
Moremen, Kelley
Merrill, Alfred H.
Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title_full Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title_fullStr Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title_full_unstemmed Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title_short Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
title_sort transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817578/
https://www.ncbi.nlm.nih.gov/pubmed/19786568
http://dx.doi.org/10.1194/jlr.M000984
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