Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

BACKGROUND: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. METHODS: Microbiology studies were performed according to standard protocols. The resist...

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Autores principales: Kasap, Murat, Fashae, Kayode, Torol, Sinem, Kolayli, Fetiye, Budak, Fatma, Vahaboglu, Haluk
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817643/
https://www.ncbi.nlm.nih.gov/pubmed/20067633
http://dx.doi.org/10.1186/1476-0711-9-1
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author Kasap, Murat
Fashae, Kayode
Torol, Sinem
Kolayli, Fetiye
Budak, Fatma
Vahaboglu, Haluk
author_facet Kasap, Murat
Fashae, Kayode
Torol, Sinem
Kolayli, Fetiye
Budak, Fatma
Vahaboglu, Haluk
author_sort Kasap, Murat
collection PubMed
description BACKGROUND: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. METHODS: Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. RESULTS: By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed bla(SHV-12 )associated to the insertion sequence, IS26. CONCLUSION: This is the first study demonstrated the occurrence of SHV-12 in Nigeria.
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spelling pubmed-28176432010-02-09 Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria Kasap, Murat Fashae, Kayode Torol, Sinem Kolayli, Fetiye Budak, Fatma Vahaboglu, Haluk Ann Clin Microbiol Antimicrob Research BACKGROUND: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. METHODS: Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. RESULTS: By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed bla(SHV-12 )associated to the insertion sequence, IS26. CONCLUSION: This is the first study demonstrated the occurrence of SHV-12 in Nigeria. BioMed Central 2010-01-12 /pmc/articles/PMC2817643/ /pubmed/20067633 http://dx.doi.org/10.1186/1476-0711-9-1 Text en Copyright ©2010 Kasap et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kasap, Murat
Fashae, Kayode
Torol, Sinem
Kolayli, Fetiye
Budak, Fatma
Vahaboglu, Haluk
Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title_full Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title_fullStr Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title_full_unstemmed Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title_short Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria
title_sort characterization of esbl (shv-12) producing clinical isolate of enterobacter aerogenes from a tertiary care hospital in nigeria
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817643/
https://www.ncbi.nlm.nih.gov/pubmed/20067633
http://dx.doi.org/10.1186/1476-0711-9-1
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